{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Meng Z"],"funding":["Tianjin Municipal Science and Technology Commission","National Natural Science Foundation of China"],"pagination":["13414"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9655393"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["23(21)"],"pubmed_abstract":["Mediator complex subunit 1 (MED1) is a coactivator of multiple transcription factors and plays a key role in regulating epidermal homeostasis as well as skin wound healing. It is unknown, however, whether it plays a role in healing oral mucosal wounds. In this study, we investigate MED1's functional effects on oral mucosal wound healing and its underlying mechanism. The epithelial-specific MED1 null (Med1<sup>epi-/-</sup>) mice were established using the <i>Cre-loxP</i> system with C57/BL6 background. A 3 mm diameter wound was made in the cheek mucosa of the 8-week-old mice. In vivo experiments were conducted using HE staining and immunostaining with Ki67 and uPAR antibodies. The in vitro study used lentiviral transduction, scratch assays, qRT-PCR, and Western blotting to reveal the underl"],"journal":["International journal of molecular sciences"],"pubmed_title":["MED1 Ablation Promotes Oral Mucosal Wound Healing via JNK Signaling Pathway."],"pmcid":["PMC9655393"],"funding_grant_id":["21JCQNJC01190","81970958","81700927"],"pubmed_authors":["Liu J","Li Z","Wei R","Guo S","Wu D","Hu L","Sui L","Meng Z"],"additional_accession":[]},"is_claimable":false,"name":"MED1 Ablation Promotes Oral Mucosal Wound Healing via JNK Signaling Pathway.","description":"Mediator complex subunit 1 (MED1) is a coactivator of multiple transcription factors and plays a key role in regulating epidermal homeostasis as well as skin wound healing. It is unknown, however, whether it plays a role in healing oral mucosal wounds. In this study, we investigate MED1's functional effects on oral mucosal wound healing and its underlying mechanism. The epithelial-specific MED1 null (Med1<sup>epi-/-</sup>) mice were established using the <i>Cre-loxP</i> system with C57/BL6 background. A 3 mm diameter wound was made in the cheek mucosa of the 8-week-old mice. In vivo experiments were conducted using HE staining and immunostaining with Ki67 and uPAR antibodies. The in vitro study used lentiviral transduction, scratch assays, qRT-PCR, and Western blotting to reveal the underl","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Nov","modification":"2025-04-25T23:34:15.18Z","creation":"2025-04-06T09:23:10.563Z"},"accession":"S-EPMC9655393","cross_references":{"pubmed":["36362197"],"doi":["10.3390/ijms232113414"]}}