biostudies-literatureMeng ZTianjin Municipal Science and Technology CommissionNational Natural Science Foundation of China13414https://www.ebi.ac.uk/biostudies/studies/S-EPMC9655393biostudies-literatureUnknown23(21)Mediator complex subunit 1 (MED1) is a coactivator of multiple transcription factors and plays a key role in regulating epidermal homeostasis as well as skin wound healing. It is unknown, however, whether it plays a role in healing oral mucosal wounds. In this study, we investigate MED1's functional effects on oral mucosal wound healing and its underlying mechanism. The epithelial-specific MED1 null (Med1^epi-/-) mice were established using the <i>Cre-loxP</i> system with C57/BL6 background. A 3 mm diameter wound was made in the cheek mucosa of the 8-week-old mice. In vivo experiments were conducted using HE staining and immunostaining with Ki67 and uPAR antibodies. The in vitro study used lentiviral transduction, scratch assays, qRT-PCR, and Western blotting to reveal the underlInternational journal of molecular sciencesMED1 Ablation Promotes Oral Mucosal Wound Healing via JNK Signaling Pathway.PMC965539321JCQNJC011908197095881700927Liu JLi ZWei RGuo SWu DHu LSui LMeng ZfalseMED1 Ablation Promotes Oral Mucosal Wound Healing via JNK Signaling Pathway.Mediator complex subunit 1 (MED1) is a coactivator of multiple transcription factors and plays a key role in regulating epidermal homeostasis as well as skin wound healing. It is unknown, however, whether it plays a role in healing oral mucosal wounds. In this study, we investigate MED1's functional effects on oral mucosal wound healing and its underlying mechanism. The epithelial-specific MED1 null (Med1^epi-/-) mice were established using the <i>Cre-loxP</i> system with C57/BL6 background. A 3 mm diameter wound was made in the cheek mucosa of the 8-week-old mice. In vivo experiments were conducted using HE staining and immunostaining with Ki67 and uPAR antibodies. The in vitro study used lentiviral transduction, scratch assays, qRT-PCR, and Western blotting to reveal the underl2022-01-01T00:00:00Z2022 Nov2025-04-25T23:34:15.18Z2025-04-06T09:23:10.563ZS-EPMC96553933636219710.3390/ijms232113414