biostudies-literatureE Sthe Scientific Research of Hunan Provincial Education Department7643https://www.ebi.ac.uk/biostudies/studies/S-EPMC9656101biostudies-literatureUnknown27(21)<i>Sabia schumanniana</i> Diels (SSD) is a plant whose stems are used in traditional folk medicine for the treatment of lumbago and arthralgia. Previous studies have revealed chemical constituents of SSD, including triterpenoids and aporphine alkaloids. Aporphine alkaloids contain a variety of active components, which might facilitate the effective treatment of lumbago and arthralgia. However, only 5-oxoaporphine (fuseine) has been discovered in SSD to date. In this study, we sought to systematically identify the aporphine alkaloids in SSD. We established a fast and reliable method for the detection and identification of these aporphine alkaloids based on ultra-high-performance liquid chromatography (UHPLC)-Q-Exactive-Orbitrap/mass spectrometry combined with parallel reaction monitoring (PMolecules (Basel, Switzerland)A Systematic Method for the Identification of Aporphine Alkaloid Constituents in <i>Sabia&amp;nbsp;schumanniana</i> Diels Using UHPLC-Q-Exactive Orbitrap/Mass Spectrometry.PMC9656101no. 19A353Liu YNE SShang ZCLi KLWu JLQin SHYan FCai WfalseA Systematic Method for the Identification of Aporphine Alkaloid Constituents in <i>Sabia&amp;nbsp;schumanniana</i> Diels Using UHPLC-Q-Exactive Orbitrap/Mass Spectrometry.<i>Sabia schumanniana</i> Diels (SSD) is a plant whose stems are used in traditional folk medicine for the treatment of lumbago and arthralgia. Previous studies have revealed chemical constituents of SSD, including triterpenoids and aporphine alkaloids. Aporphine alkaloids contain a variety of active components, which might facilitate the effective treatment of lumbago and arthralgia. However, only 5-oxoaporphine (fuseine) has been discovered in SSD to date. In this study, we sought to systematically identify the aporphine alkaloids in SSD. We established a fast and reliable method for the detection and identification of these aporphine alkaloids based on ultra-high-performance liquid chromatography (UHPLC)-Q-Exactive-Orbitrap/mass spectrometry combined with parallel reaction monitoring (P2022-01-01T00:00:00Z2022 Nov2025-04-22T19:01:31.4Z2025-04-06T02:40:51.754ZS-EPMC96561013636447910.3390/molecules27217643