biostudies-literatureQin HFundamental Research Funds for the Central UniversitiesHeilongjiang Touyan Innovation Team Programthe National Natural Science Foundation of China13102https://www.ebi.ac.uk/biostudies/studies/S-EPMC9656282biostudies-literatureUnknown23(21)In Brassicaceae, the papillary cells of the stigma are the primary site of the self-incompatibility (SI) responses. SI preserves the genetic diversity by selectively rejecting irrelevant or incompatible pollen, thus promoting cross fertilization and species fitness. Mechanisms that regulate SI responses in <i>Brassica</i> have been studied mainly on the mature stigma that often undermines how stigma papillary cells attain the state of SI during development. To understand this, we integrated PacBio SMRT-seq with Illumina RNA-seq to construct a de novo full-length transcriptomic database for different stages of stigma development in ornamental kale. A total of 48,800 non-redundant transcripts, 31,269 novel transcripts, 24,015 genes, 13,390 alternative splicing, 22,389 simple sequence repeatsInternational journal of molecular sciencesFatty Acid Biosynthesis Pathways Are Downregulated during Stigma Development and Are Critical during Self-Incompatible Responses in Ornamental Kale.PMC9656282(Tree Genetics and Breeding Innovation Team)2572022AW6631870300Li HYao KAbhinandan KZhao RQin HShi JXun BLan XLi MWu YfalseFatty Acid Biosynthesis Pathways Are Downregulated during Stigma Development and Are Critical during Self-Incompatible Responses in Ornamental Kale.In Brassicaceae, the papillary cells of the stigma are the primary site of the self-incompatibility (SI) responses. SI preserves the genetic diversity by selectively rejecting irrelevant or incompatible pollen, thus promoting cross fertilization and species fitness. Mechanisms that regulate SI responses in <i>Brassica</i> have been studied mainly on the mature stigma that often undermines how stigma papillary cells attain the state of SI during development. To understand this, we integrated PacBio SMRT-seq with Illumina RNA-seq to construct a de novo full-length transcriptomic database for different stages of stigma development in ornamental kale. A total of 48,800 non-redundant transcripts, 31,269 novel transcripts, 24,015 genes, 13,390 alternative splicing, 22,389 simple sequence repeats2022-01-01T00:00:00Z2022 Oct2025-04-22T12:31:21.933Z2024-11-10T02:35:01.388ZS-EPMC96562823636188710.3390/ijms232113102