biostudies-literatureWang XJiangsu Provincial Key Research and Development ProgramJiangsu Agriculture Science and Technology Innovation FundPriority Academic Program Development of Jiangsu Higher Education InstitutionsNational Natural Science Foundation of ChinaQing Lan Project of Jiangsu ProvinceNatural Science Foundation of Jiangsu Province3330https://www.ebi.ac.uk/biostudies/studies/S-EPMC9656536biostudies-literatureUnknown11(21)Two exopolysaccharide fractions (GL1-E1 and GL1-E2) of Lacticaseibacillus paracasei GL1 were isolated with the molecular weights of 3.9 × 105 Da and 8.2 × 105 Da, respectively. Both fractions possessed mannose, glucose, and galactose in molar ratios of 1.16:1.00:0.1, and 3.81:1.00:0.12, respectively. A structural arrangement of two fractions was proposed by methylation, one-dimensional and two-dimensional nuclear magnetic resonance experiments. The backbone of GL1-E1 consisted of →4)-α-D-Glcp(1→, →3,4)-α-D-Manp(1→, →3,6)-α-D-Manp(1→, →6)-α-D-Manp(1→, and →6)-α-D-Galp(1→ with α-D-Glcp at branching point. The backbone of GL1-E2 consisted of →4)-α-D-Glcp(1→, →3,4)-α-DFoods (Basel, Switzerland)Characterization and Immunological Activity of Exopolysaccharide from Lacticaseibacillus paracasei GL1 Isolated from Tibetan Kefir Grains.PMC9656536PAPDQLPJCX(20)3043BK202030531871771BK20201320XZ-SZ202032U1903108Tian JLi WRui XTang NZhang XDong MWang XZhang QfalseCharacterization and Immunological Activity of Exopolysaccharide from Lacticaseibacillus paracasei GL1 Isolated from Tibetan Kefir Grains.Two exopolysaccharide fractions (GL1-E1 and GL1-E2) of Lacticaseibacillus paracasei GL1 were isolated with the molecular weights of 3.9 × 105 Da and 8.2 × 105 Da, respectively. Both fractions possessed mannose, glucose, and galactose in molar ratios of 1.16:1.00:0.1, and 3.81:1.00:0.12, respectively. A structural arrangement of two fractions was proposed by methylation, one-dimensional and two-dimensional nuclear magnetic resonance experiments. The backbone of GL1-E1 consisted of →4)-α-D-Glcp(1→, →3,4)-α-D-Manp(1→, →3,6)-α-D-Manp(1→, →6)-α-D-Manp(1→, and →6)-α-D-Galp(1→ with α-D-Glcp at branching point. The backbone of GL1-E2 consisted of →4)-α-D-Glcp(1→, →3,4)-α-D2022-01-01T00:00:00Z2022 Oct2025-04-22T12:25:05.681Z2025-04-06T00:21:03.952ZS-EPMC96565363635994210.3390/foods11213330