{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Zeng C"],"funding":["Guizhou Science and Technology Support Program","Natural Science Foundation of China","Cultivation project plan of new seedling cultivation and innovation exploration special project of Zunyi Medical University, and Platform talents of Qiankehe","Talents of Guizhou Science and Technology Cooperation Platform","Zunyi Science and Technology Plan","the Excellent Youth Scientific Talents of Guizhou","Guizhou Science and Technology Plan","Platform talents of Qiankehe"],"pagination":["7237"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9656901"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["27(21)"],"pubmed_abstract":["Hypochlorous acid (HOCl) is a reactive substance that reacts with most biomolecules and is essential in physiological and pathological processes. Abnormally elevated HOCl levels may cause inflammation and other disease responses. To further understand its key role in inflammation, HOCl must be detected in situ. Here, we designed a hydroxytricyanopyrrole-based small-molecule fluorescent probe (HTCP-NTC) to monitor and identify trace amounts of HOCl in biological systems. In the presence of HOCl, HTCP-NTC released hydroxyl groups that emit strong fluorescence covering a wide wavelength range from the visible to near-infrared region owing to the resumption of the intramolecular charge transfer process. Additionally, HTCP-NTC demonstrated a 202-fold fluorescence enhancement accompanied by a large Stokes shift and a low detection limit (21.7 nM). Furthermore, HTCP-NTC provided a rapid response to HOCl within 18 s, allowing real-time monitoring of intracellular HOCl. HTCP-NTC exhibited rapid kinetics and biocompatibility, allowing effective monitoring of the exogenous and endogenous HOCl fluctuations in living cells. Finally, based on fluorescence imaging, HTCP-NTC is a potential method for understanding the relationship between inflammation and HOCl."],"journal":["Molecules (Basel, Switzerland)"],"pubmed_title":["A Hydroxytricyanopyrrole-Based Fluorescent Probe for Sensitive and Selective Detection of Hypochlorous Acid."],"pmcid":["PMC9656901"],"funding_grant_id":["(2020)4Y158","(2018) No. 5772-030","81360471","(2021)5638","2018)5772-053","82060626","Zunshikehe HZ Zi (2020)46","(2020)4104","Qianke He Foundation-ZK (2021) General 035"],"pubmed_authors":["Lv J","Zeng C","Li H","Chen Z","Yang M","Gao J","Yuan Z"],"additional_accession":[]},"is_claimable":false,"name":"A Hydroxytricyanopyrrole-Based Fluorescent Probe for Sensitive and Selective Detection of Hypochlorous Acid.","description":"Hypochlorous acid (HOCl) is a reactive substance that reacts with most biomolecules and is essential in physiological and pathological processes. Abnormally elevated HOCl levels may cause inflammation and other disease responses. To further understand its key role in inflammation, HOCl must be detected in situ. Here, we designed a hydroxytricyanopyrrole-based small-molecule fluorescent probe (HTCP-NTC) to monitor and identify trace amounts of HOCl in biological systems. In the presence of HOCl, HTCP-NTC released hydroxyl groups that emit strong fluorescence covering a wide wavelength range from the visible to near-infrared region owing to the resumption of the intramolecular charge transfer process. Additionally, HTCP-NTC demonstrated a 202-fold fluorescence enhancement accompanied by a large Stokes shift and a low detection limit (21.7 nM). Furthermore, HTCP-NTC provided a rapid response to HOCl within 18 s, allowing real-time monitoring of intracellular HOCl. HTCP-NTC exhibited rapid kinetics and biocompatibility, allowing effective monitoring of the exogenous and endogenous HOCl fluctuations in living cells. Finally, based on fluorescence imaging, HTCP-NTC is a potential method for understanding the relationship between inflammation and HOCl.","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Oct","modification":"2025-04-22T19:08:16.34Z","creation":"2025-04-06T02:38:37.278Z"},"accession":"S-EPMC9656901","cross_references":{"pubmed":["36364062"],"doi":["10.3390/molecules27217237"]}}