biostudies-literatureBlankenship LCPRIT Core Facility AwardT.E.A.L.®’s 2021 Medical Research ProgramThriveWell Cancer FoundationInstitutional Research GrantsNCI NIH HHSAmerican Cancer Society Research Scholar GrantsNational Institutes of HealthNIH HHSCancer Center at UT Health San AntonioInstitutional CPRIT Research Training AwardNIH Shared Instrument5400https://www.ebi.ac.uk/biostudies/studies/S-EPMC9657203biostudies-literatureUnknown14(21)Endometrial cancer (EC) is the fourth most common cancer in women, and half of the endometrioid EC (EEC) cases are attributable to obesity. However, the underlying mechanism(s) of obesity-driven EEC remain(s) unclear. In this study, we examined whether LIF signaling plays a role in the obesity-driven progression of EEC. RNA-seq analysis of EEC cells stimulated by adipose conditioned medium (ADP-CM) showed upregulation of LIF/LIFR-mediated signaling pathways including JAK/STAT and interleukin pathways. Immunohistochemistry analysis of normal and EEC tissues collected from obese patients revealed that LIF expression is upregulated in EEC tissues compared to the normal endometrium. Treatment of both primary and established EEC cells with ADP-CM increased the expression of LIF and its receptorCancersInhibition of LIFR Blocks Adiposity-Driven Endometrioid Endometrial Cancer Growth.PMC9657203170722-44096IRG-21-147-01-IRGNIH-NCI P30 CA054174R50 CA265339RP170345P30 CA054174S10 OD0218051S10OD021805-01R01 CA267893170711-44096RP160732R01CA267893132931-RSG-18-187-01-TBGKonda SSareddy GRZheng SVadlamudi RKTekmal RRKost ERViswanadhapalli SYang XRamasamy KLiu ZLai ZBlankenship LPratap UPNair HBAltwegg KAChen YSanthamma BValente PTfalseInhibition of LIFR Blocks Adiposity-Driven Endometrioid Endometrial Cancer Growth.Endometrial cancer (EC) is the fourth most common cancer in women, and half of the endometrioid EC (EEC) cases are attributable to obesity. However, the underlying mechanism(s) of obesity-driven EEC remain(s) unclear. In this study, we examined whether LIF signaling plays a role in the obesity-driven progression of EEC. RNA-seq analysis of EEC cells stimulated by adipose conditioned medium (ADP-CM) showed upregulation of LIF/LIFR-mediated signaling pathways including JAK/STAT and interleukin pathways. Immunohistochemistry analysis of normal and EEC tissues collected from obese patients revealed that LIF expression is upregulated in EEC tissues compared to the normal endometrium. Treatment of both primary and established EEC cells with ADP-CM increased the expression of LIF and its receptor2022-01-01T00:00:00Z2022 Nov2025-04-04T00:36:47.974Z2025-04-04T00:36:47.974ZS-EPMC96572033635881810.3390/cancers14215400