biostudies-literatureHaroonNational Natural Science Foundation of China13660https://www.ebi.ac.uk/biostudies/studies/S-EPMC9657995biostudies-literatureUnknown23(21)The longevity phenomenon is entirely controlled by the insulin signaling pathway (IIS-pathway). Both vertebrates and invertebrates have IIS-pathways that are comparable to one another, though no one has previously described de novo transcriptome assembly of IIS-pathway-associated genes in termites. In this research, we analyzed the transcriptomes of both reproductive (primary kings "PK" and queens "PQ", secondary worker reproductive kings "SWRK" and queens "SWRQ") and non-reproductive (male "WM" and female "WF" workers) castes of the subterranean termite <i>Reticulitermes chinensis</i>. The goal was to identify the genes responsible for longevity in the reproductive and non-reproductive castes. Through transcriptome analysis, we annotated 103,589,264 sequence reads and 184,436 (7G) unigeneInternational journal of molecular sciences<i>De Novo</i> Transcriptome Assembly and Analysis of Longevity Genes Using Subterranean Termite (<i>Reticulitermes chinensis</i>) Castes.PMC965799531870389Su XHSu JHaroonXing LXLi YXYe CXNabi Gfalse<i>De Novo</i> Transcriptome Assembly and Analysis of Longevity Genes Using Subterranean Termite (<i>Reticulitermes chinensis</i>) Castes.The longevity phenomenon is entirely controlled by the insulin signaling pathway (IIS-pathway). Both vertebrates and invertebrates have IIS-pathways that are comparable to one another, though no one has previously described de novo transcriptome assembly of IIS-pathway-associated genes in termites. In this research, we analyzed the transcriptomes of both reproductive (primary kings "PK" and queens "PQ", secondary worker reproductive kings "SWRK" and queens "SWRQ") and non-reproductive (male "WM" and female "WF" workers) castes of the subterranean termite <i>Reticulitermes chinensis</i>. The goal was to identify the genes responsible for longevity in the reproductive and non-reproductive castes. Through transcriptome analysis, we annotated 103,589,264 sequence reads and 184,436 (7G) unigene2022-01-01T00:00:00Z2022 Nov2025-04-25T23:26:08.506Z2025-04-06T09:21:22.642ZS-EPMC96579953636244710.3390/ijms232113660