biostudies-literatureUnknown27(21)Jivkova DThe current study examines the desiccation-resistant <i>Ramlibacter tataouinensis</i> TTB310^T as a model organism for the production of novel exopolysaccharides and their structural features. This bacterium is able to produce dividing forms of cysts which synthesize cell-bound exopolysaccharide. Initial experiments were conducted on the enrichment of cyst biomass for exopolysaccharide production under batch-fed conditions in a pilot-scale bioreactor, with lactate as the source of carbon and energy. The optimized medium produced significant quantities of exopolysaccharide in a single growth phase, since the production of exopolysaccharide took place during the division of the cysts. The exopolysaccharide layer was extracted from the cysts using a modified trichloroacetic acid metMolecules (Basel, Switzerland)7172https://www.ebi.ac.uk/biostudies/studies/S-EPMC9658432biostudies-literatureProduction and Characterization of a Novel Exopolysaccharide from <i>Ramlibacter tataouinensis</i>.PMC9658432Harir MFochesato SHertkorn NJivkova DHeyraud ABerthomieu CSantaella CAchouak WHeulin TSathiyanarayanan GSchmitt-Kopplin PSanhaji GfalseProduction and Characterization of a Novel Exopolysaccharide from <i>Ramlibacter tataouinensis</i>.The current study examines the desiccation-resistant <i>Ramlibacter tataouinensis</i> TTB310^T as a model organism for the production of novel exopolysaccharides and their structural features. This bacterium is able to produce dividing forms of cysts which synthesize cell-bound exopolysaccharide. Initial experiments were conducted on the enrichment of cyst biomass for exopolysaccharide production under batch-fed conditions in a pilot-scale bioreactor, with lactate as the source of carbon and energy. The optimized medium produced significant quantities of exopolysaccharide in a single growth phase, since the production of exopolysaccharide took place during the division of the cysts. The exopolysaccharide layer was extracted from the cysts using a modified trichloroacetic acid met2022-01-01T00:00:00Z2022 Oct2025-04-22T18:58:44.696Z2024-11-05T21:59:52.127ZS-EPMC96584323636400310.3390/molecules27217172