biostudies-literatureUnknown23(21)Mroczek JMineralization-competent cells like osteoblasts and chondrocytes release matrix vesicles (MVs) which accumulate Ca²⁺ and P_i, creating an optimal environment for apatite formation. The mineralization process requires the involvement of proteins, such as annexins (Anx) and tissue-nonspecific alkaline phosphatase (TNAP), as well as low molecular-weight compounds. Apigenin, a flavonoid compound, has been reported to affect bone metabolism, but there are doubts about its mechanism of action under physiological and pathological conditions. In this report, apigenin potency to modulate annexin A6 (AnxA6)- and TNAP-mediated osteoblast mineralization was explored using three cell lines: human fetal osteoblastic hFOB 1.19, human osteosarcoma Saos-2, and human coronary artery smoInternational journal of molecular sciences13179https://www.ebi.ac.uk/biostudies/studies/S-EPMC9658728biostudies-literatureApigenin Modulates AnxA6- and TNAP-Mediated Osteoblast Mineralization.PMC9658728Suski SStrzelecka-Kiliszek AMroczek JBiesaga MPikula SWeremiejczyk LfalseApigenin Modulates AnxA6- and TNAP-Mediated Osteoblast Mineralization.Mineralization-competent cells like osteoblasts and chondrocytes release matrix vesicles (MVs) which accumulate Ca²⁺ and P_i, creating an optimal environment for apatite formation. The mineralization process requires the involvement of proteins, such as annexins (Anx) and tissue-nonspecific alkaline phosphatase (TNAP), as well as low molecular-weight compounds. Apigenin, a flavonoid compound, has been reported to affect bone metabolism, but there are doubts about its mechanism of action under physiological and pathological conditions. In this report, apigenin potency to modulate annexin A6 (AnxA6)- and TNAP-mediated osteoblast mineralization was explored using three cell lines: human fetal osteoblastic hFOB 1.19, human osteosarcoma Saos-2, and human coronary artery smo2022-01-01T00:00:00Z2022 Oct2025-04-25T23:36:21.821Z2025-04-06T09:23:45.168ZS-EPMC96587283636196510.3390/ijms232113179