biostudies-literatureUnknown45(5)Walweel K<h4>Introduction</h4>Organs procured following brain stem death (BSD) are the main source of organ grafts for transplantation. However, BSD is associated with inflammatory responses that may damage the organ and affect both the quantity and quality of organs available for transplant. Therefore, we aimed to investigate plasma and bronchoalveolar lavage (BAL) pro-inflammatory cytokine profiles and cardiovascular physiology in a clinically relevant 6-h ovine model of BSD.<h4>Methods</h4>Twelve healthy female sheep (37-42 Kg) were anaesthetized and mechanically ventilated prior to undergoing BSD induction and then monitored for 6 h. Plasma and BAL endothelin-1 and cytokines (IL-1β, 6, 8 and tumour necrosis factor alpha (TNF-α)) were assessed by ELISA. Differential white blood cell counts were Biomedical journal776-787https://www.ebi.ac.uk/biostudies/studies/S-EPMC9661508biostudies-literatureBrain stem death induces pro-inflammatory cytokine production and cardiac dysfunction in sheep model.PMC9661508Colombo SMSkeggs KWells MAObonyo NGBartnikowski NJFraser JFBouquet MSee Hoe LEStevenson AKHyslop KMarshall LPimenta LPBoon ACWalweel KMcGiffin DCPassmore MRJames LNSuen JYBisht KMacdonald PSPrabhu ABlack DMPlatts DGDiab SDPedersen SEfalseBrain stem death induces pro-inflammatory cytokine production and cardiac dysfunction in sheep model.<h4>Introduction</h4>Organs procured following brain stem death (BSD) are the main source of organ grafts for transplantation. However, BSD is associated with inflammatory responses that may damage the organ and affect both the quantity and quality of organs available for transplant. Therefore, we aimed to investigate plasma and bronchoalveolar lavage (BAL) pro-inflammatory cytokine profiles and cardiovascular physiology in a clinically relevant 6-h ovine model of BSD.<h4>Methods</h4>Twelve healthy female sheep (37-42 Kg) were anaesthetized and mechanically ventilated prior to undergoing BSD induction and then monitored for 6 h. Plasma and BAL endothelin-1 and cytokines (IL-1β, 6, 8 and tumour necrosis factor alpha (TNF-α)) were assessed by ELISA. Differential white blood cell counts were 2022-01-01T00:00:00Z2022 Oct2025-04-25T23:27:06.99Z2025-04-06T09:21:31.467ZS-EPMC96615083466621910.1016/j.bj.2021.10.007