{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Strazza M"],"funding":["NCRR NIH HHS","NIAID NIH HHS","NCI NIH HHS","NIH HHS"],"pagination":["380-390"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9664140"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["27"],"pubmed_abstract":["The transmembrane adaptor phosphoprotein associated with glycosphingolipid-enriched microdomains 1 (PAG) is phosphorylated in T cells downstream of PD-1 signaling and contributes to the resulting functional inhibition of multiple cellular processes. Furthermore, PAG expression is negatively correlated with survival in multiple human tumors and is a driver of murine tumor growth and immune evasion. Here we develop an antibody that targets the extracellular domain of human PAG, with cross-reactivity to murine PAG. We demonstrate that this antibody binds to extracellular PAG on intact cells and affects T cell activation. Finally, we show that administration of anti-PAG monoclonal antibody in combination with anti-PD-1 antibody to mice bearing MC38 tumors limited tumor growth and enhanced T ce"],"journal":["Molecular therapy. Methods & clinical development"],"pubmed_title":["Neutralization of the adaptor protein PAG by monoclonal antibody limits murine tumor growth."],"pmcid":["PMC9664140"],"funding_grant_id":["S10 RR027050","S10 OD020056","R21 CA231277","R01 AI150597","R01 AI125640","F30 CA271624"],"pubmed_authors":["Mor A","Adam K","Azoulay-Alfaguter I","Strazza M","Moore EK"],"additional_accession":[]},"is_claimable":false,"name":"Neutralization of the adaptor protein PAG by monoclonal antibody limits murine tumor growth.","description":"The transmembrane adaptor phosphoprotein associated with glycosphingolipid-enriched microdomains 1 (PAG) is phosphorylated in T cells downstream of PD-1 signaling and contributes to the resulting functional inhibition of multiple cellular processes. Furthermore, PAG expression is negatively correlated with survival in multiple human tumors and is a driver of murine tumor growth and immune evasion. Here we develop an antibody that targets the extracellular domain of human PAG, with cross-reactivity to murine PAG. We demonstrate that this antibody binds to extracellular PAG on intact cells and affects T cell activation. Finally, we show that administration of anti-PAG monoclonal antibody in combination with anti-PD-1 antibody to mice bearing MC38 tumors limited tumor growth and enhanced T ce","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Dec","modification":"2025-07-13T03:04:52.092Z","creation":"2025-04-05T11:36:03.442Z"},"accession":"S-EPMC9664140","cross_references":{"pubmed":["36419471"],"doi":["10.1016/j.omtm.2022.10.012"]}}