{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Wienberg F"],"funding":["Bundesministerium für Bildung und Forschung","Rheinische Friedrich-Wilhelms-Universität Bonn"],"pagination":["144"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9666576"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["12(1)"],"pubmed_abstract":["Due to the health-promoting effects and functional properties of inulin-type fructooligosaccharides (I-FOS), the global market for I-FOS is constantly growing. Hence, there is a continuing demand for new, efficient biotechnological approaches for I-FOS production. In this work, crude inulosucrase InuGB-V3 from Lactobacillus gasseri DSM 20604 was used to synthesize I-FOS from sucrose. Supplementation with 1 mM CaCl<sub>2</sub>, a pH of 3.5-5.5, and an incubation temperature of 40 °C were found to be optimal production parameters at which crude inulosucrase showed high conversion rates, low sucrose hydrolysis, and excellent stability over 4 days. The optimal process conditions were employed in cell-free bioconversion reactions. By elevating the substrate concentration from 570 to 800 g L<sup"],"journal":["AMB Express"],"pubmed_title":["High-yield production and purification of prebiotic inulin-type fructooligosaccharides."],"pmcid":["PMC9666576"],"funding_grant_id":["FKZ 031B1054A"],"pubmed_authors":["Wienberg F","Deppenmeier U","Hovels M"],"additional_accession":[]},"is_claimable":false,"name":"High-yield production and purification of prebiotic inulin-type fructooligosaccharides.","description":"Due to the health-promoting effects and functional properties of inulin-type fructooligosaccharides (I-FOS), the global market for I-FOS is constantly growing. Hence, there is a continuing demand for new, efficient biotechnological approaches for I-FOS production. In this work, crude inulosucrase InuGB-V3 from Lactobacillus gasseri DSM 20604 was used to synthesize I-FOS from sucrose. Supplementation with 1 mM CaCl<sub>2</sub>, a pH of 3.5-5.5, and an incubation temperature of 40 °C were found to be optimal production parameters at which crude inulosucrase showed high conversion rates, low sucrose hydrolysis, and excellent stability over 4 days. The optimal process conditions were employed in cell-free bioconversion reactions. By elevating the substrate concentration from 570 to 800 g L<sup","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Nov","modification":"2025-04-19T13:25:07.213Z","creation":"2025-04-19T13:25:07.213Z"},"accession":"S-EPMC9666576","cross_references":{"pubmed":["36380213"],"doi":["10.1186/s13568-022-01485-9"]}}