{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Duan S"],"funding":["Key Laboratory of Highway Construction and Maintenance Technology in the Loess Region of Shanxi Transportation Research Institute"],"pagination":["38-47"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9668804"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["50(1)"],"pubmed_abstract":["<h4>Purpose</h4>We first developed a 4-1BB-targeted optical probe, named IRDye-680RD-4-1BB mAb (monoclonal antibody), and evaluated its value for the detection of 4-1BB<sup>+</sup> activated T cells in vivo as well as the diagnosis of rheumatoid arthritis (RA) in an adjuvant-induced arthritis (AIA) mouse model.<h4>Methods</h4>The 4-1BB expression pattern was analysed by flow cytometry and immunofluorescence (IF) staining. The 4-1BB mAb was conjugated with IRDye-680RD NHS ester, and characterized via fluorescence spectrum. A cell-binding assay was also performed to assess the interaction of this probe with activated and naïve murine T cells. Longitudinal near-infrared fluorescence (NIRF) imaging of the probe was performed at 6, 24, 48, 72, and 96 h after probe administration.<h4>Results</h4"],"journal":["European journal of nuclear medicine and molecular imaging"],"pubmed_title":["Fluorophore-conjugated 4-1BB antibody enables early detection of T-cell responses in inflammatory arthritis via NIRF imaging."],"pmcid":["PMC9668804"],"funding_grant_id":["KYCX2019-09"],"pubmed_authors":["Xia Y","Li C","Xiao Z","Li P","Zhang M","Zhang X","Chen W","Duan S","Jing C","Wang W","Han C","Wang Y","Dong B"],"additional_accession":[]},"is_claimable":false,"name":"Fluorophore-conjugated 4-1BB antibody enables early detection of T-cell responses in inflammatory arthritis via NIRF imaging.","description":"<h4>Purpose</h4>We first developed a 4-1BB-targeted optical probe, named IRDye-680RD-4-1BB mAb (monoclonal antibody), and evaluated its value for the detection of 4-1BB<sup>+</sup> activated T cells in vivo as well as the diagnosis of rheumatoid arthritis (RA) in an adjuvant-induced arthritis (AIA) mouse model.<h4>Methods</h4>The 4-1BB expression pattern was analysed by flow cytometry and immunofluorescence (IF) staining. The 4-1BB mAb was conjugated with IRDye-680RD NHS ester, and characterized via fluorescence spectrum. A cell-binding assay was also performed to assess the interaction of this probe with activated and naïve murine T cells. Longitudinal near-infrared fluorescence (NIRF) imaging of the probe was performed at 6, 24, 48, 72, and 96 h after probe administration.<h4>Results</h4","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Dec","modification":"2025-04-19T18:50:59.217Z","creation":"2025-04-19T18:50:59.217Z"},"accession":"S-EPMC9668804","cross_references":{"pubmed":["36069957"],"doi":["10.1007/s00259-022-05946-y"]}}