{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["14(4)"],"submitter":["Wang D"],"pubmed_abstract":["<h4>Objective</h4>Due to the complicated compounds and the synergistic effect of multi-compounds, the quality control and assessment of Chinese materia medica (CMM) encounters a great challenge about how to identify the key compounds, which are directly correlated with its efficacy and safety. On the guidance of study on quality marker (Q-Marker), identification of Q-Markers was performed from Hedan Tablet (HDT) by the aid of the \"spider-web\" mode and hepatotoxicity evaluation derived from our previous researches and literatures.<h4>Methods</h4>By the established ultra performance liquid chromatography with photodiode array detector (UPLC-PDA) method, online UPLC-DPPH· and offline antioxidant assay, 21 candidate compounds of HDT were systematically investigated and comprehensively evaluated by the \"spider-web\" mode for them properties of Q-Marker based on \"content-stability-activity\". In addition, the Q-Markers related with hepatotoxicity based on our previous researches and literatures were identified.<h4>Results</h4>Salvianolic acid B (SaB), quercetin-3-<i>O</i>-glucuronide (Qug), isoquercitrin (IQ) and hyperoside (Hyp) were adopted as the preferable Q-Markers of HDT according to the shaded area (A) of tested compounds in \"spider-web\" mode. Psoralen (Ps), isopsoralen (IP), psoralenoside (PO) and isopsoralenoside (IPO) were also strongly recommended as Q-Markers closely related with safety by considering hepatotoxicity of the accumulated Ps and IP and conversion between glycoside (PO and IPO) and aglycone (Ps and IP).<h4>Conclusion</h4>This study provided scientific evidence for quality control and assessment of HDT, and also provided a meaningful reference for application of Q-Markers in CMM."],"journal":["Chinese herbal medicines"],"pagination":["612-621"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9669348"],"repository":["biostudies-literature"],"pubmed_title":["Identification of Q-Markers from Hedan Tablet by employing \"spider-web\" mode and taking compounds' hepatotoxicity into account."],"pmcid":["PMC9669348"],"pubmed_authors":["Yang J","Feng X","Wang D","Zhou W","Liu C","Zeng Y","Ding J","Wang Y","Chai X"],"additional_accession":[]},"is_claimable":false,"name":"Identification of Q-Markers from Hedan Tablet by employing \"spider-web\" mode and taking compounds' hepatotoxicity into account.","description":"<h4>Objective</h4>Due to the complicated compounds and the synergistic effect of multi-compounds, the quality control and assessment of Chinese materia medica (CMM) encounters a great challenge about how to identify the key compounds, which are directly correlated with its efficacy and safety. On the guidance of study on quality marker (Q-Marker), identification of Q-Markers was performed from Hedan Tablet (HDT) by the aid of the \"spider-web\" mode and hepatotoxicity evaluation derived from our previous researches and literatures.<h4>Methods</h4>By the established ultra performance liquid chromatography with photodiode array detector (UPLC-PDA) method, online UPLC-DPPH· and offline antioxidant assay, 21 candidate compounds of HDT were systematically investigated and comprehensively evaluated by the \"spider-web\" mode for them properties of Q-Marker based on \"content-stability-activity\". In addition, the Q-Markers related with hepatotoxicity based on our previous researches and literatures were identified.<h4>Results</h4>Salvianolic acid B (SaB), quercetin-3-<i>O</i>-glucuronide (Qug), isoquercitrin (IQ) and hyperoside (Hyp) were adopted as the preferable Q-Markers of HDT according to the shaded area (A) of tested compounds in \"spider-web\" mode. Psoralen (Ps), isopsoralen (IP), psoralenoside (PO) and isopsoralenoside (IPO) were also strongly recommended as Q-Markers closely related with safety by considering hepatotoxicity of the accumulated Ps and IP and conversion between glycoside (PO and IPO) and aglycone (Ps and IP).<h4>Conclusion</h4>This study provided scientific evidence for quality control and assessment of HDT, and also provided a meaningful reference for application of Q-Markers in CMM.","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Oct","modification":"2025-04-21T14:15:42.827Z","creation":"2025-04-21T14:15:42.827Z"},"accession":"S-EPMC9669348","cross_references":{"pubmed":["36405066"],"doi":["10.1016/j.chmed.2021.08.007"]}}