{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Diffendall GM"],"funding":["ERC AdG PlasmoSilencing","Agence Nationale de Recherche","ERC StG PlasmoEpiRNA"],"pagination":["e202201577"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9670795"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["6(1)"],"pubmed_abstract":["Non-coding RNAs (ncRNAs) are emerging regulators of immune evasion and transmission of <i>Plasmodium falciparum</i> RUF6 is an ncRNA gene family that is transcribed by RNA polymerase III but actively regulates the Pol II-transcribed <i>var</i> virulence gene family. Understanding how RUF6 ncRNA connects to downstream effectors is lacking. We developed an RNA-directed proteomic discovery (ChIRP-MS) protocol to identify in vivo RUF6 ncRNA-protein interactions. The RUF6 ncRNA interactome was purified with biotinylated antisense oligonucleotides. Quantitative label-free mass spectrometry identified several unique proteins linked to gene transcription including RNA Pol II subunits, nucleosome assembly proteins, and a homologue of DEAD box helicase 5 (DDX5). Affinity purification of Pf-DDX5 iden"],"journal":["Life science alliance"],"pubmed_title":["Discovery of RUF6 ncRNA-interacting proteins involved in <i>P. falciparum</i> immune evasion."],"pmcid":["PMC9670795"],"funding_grant_id":["ANR-11 LabEx -0024-01"],"pubmed_authors":["Dingli F","Loew D","Scherf A","Baumgarten S","Barcons-Simon A","Diffendall GM"],"additional_accession":[]},"is_claimable":false,"name":"Discovery of RUF6 ncRNA-interacting proteins involved in <i>P. falciparum</i> immune evasion.","description":"Non-coding RNAs (ncRNAs) are emerging regulators of immune evasion and transmission of <i>Plasmodium falciparum</i> RUF6 is an ncRNA gene family that is transcribed by RNA polymerase III but actively regulates the Pol II-transcribed <i>var</i> virulence gene family. Understanding how RUF6 ncRNA connects to downstream effectors is lacking. We developed an RNA-directed proteomic discovery (ChIRP-MS) protocol to identify in vivo RUF6 ncRNA-protein interactions. The RUF6 ncRNA interactome was purified with biotinylated antisense oligonucleotides. Quantitative label-free mass spectrometry identified several unique proteins linked to gene transcription including RNA Pol II subunits, nucleosome assembly proteins, and a homologue of DEAD box helicase 5 (DDX5). Affinity purification of Pf-DDX5 iden","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Jan","modification":"2026-05-28T00:51:40.94Z","creation":"2024-11-14T23:18:57.648Z"},"accession":"S-EPMC9670795","cross_references":{"pubmed":["36379669"],"doi":["10.26508/lsa.202201577"]}}