{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["28(12)"],"submitter":["Wang J"],"pubmed_abstract":["Box C/D RNAs guide site-specific 2'-O-methylation of RNAs in archaea and eukaryotes. The defining feature of methylation guide RNAs is two sets of box C and D motifs that form kink-turn structures specifically recognized by L7Ae family proteins. Here, we engineered a new type of methylation guide that lacks C/D motifs and requires no L7Ae for assembly and function. We determined a crystal structure of a bipartite C/D-free guide RNA in complex with Nop5, fibrillarin and substrate in the active form at 2.2 Å resolution. The stems of new guide RNAs functionally replace C/D motifs in Nop5 binding, precisely placing the substrate for site-specific modification. We also found that the bipartite architecture and association of L7Ae with C/D motifs enhance modification when association of guide RN"],"journal":["RNA (New York, N.Y.)"],"pagination":["1597-1605"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9670817"],"repository":["biostudies-literature"],"pubmed_title":["Methylation guide RNAs without box C/D motifs."],"pmcid":["PMC9670817"],"pubmed_authors":["Yang Z","Wang J","Ye K"],"additional_accession":[]},"is_claimable":false,"name":"Methylation guide RNAs without box C/D motifs.","description":"Box C/D RNAs guide site-specific 2'-O-methylation of RNAs in archaea and eukaryotes. The defining feature of methylation guide RNAs is two sets of box C and D motifs that form kink-turn structures specifically recognized by L7Ae family proteins. Here, we engineered a new type of methylation guide that lacks C/D motifs and requires no L7Ae for assembly and function. We determined a crystal structure of a bipartite C/D-free guide RNA in complex with Nop5, fibrillarin and substrate in the active form at 2.2 Å resolution. The stems of new guide RNAs functionally replace C/D motifs in Nop5 binding, precisely placing the substrate for site-specific modification. We also found that the bipartite architecture and association of L7Ae with C/D motifs enhance modification when association of guide RN","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Dec","modification":"2025-05-29T21:47:14.735Z","creation":"2025-05-29T21:47:14.735Z"},"accession":"S-EPMC9670817","cross_references":{"pubmed":["36127125"],"doi":["10.1261/rna.079379.122"]}}