biostudies-literatureBertrand BPNIAID NIH HHSNCI NIH HHSNIGMS NIH HHSe0042822https://www.ebi.ac.uk/biostudies/studies/S-EPMC9670962biostudies-literatureUnknown90(11)Biofilms are bacterial communities characterized by antibiotic tolerance. Staphylococcus aureus is a leading cause of biofilm infections on medical devices, including prosthetic joints, which represent a significant health care burden. The major leukocyte infiltrate associated with S. aureus prosthetic joint infection (PJI) is granulocytic myeloid-derived suppressor cells (G-MDSCs), which produce IL-10 to promote biofilm persistence by inhibiting monocyte and macrophage proinflammatory activity. To determine how S. aureus biofilm responds to G-MDSCs and macrophages, biofilms were cocultured with either leukocyte population followed by RNA sequencing. Several genes involved in fermentative pathways were significantly upregulated in S. aureus biofilm following G-MDSC coculture, including forInfection and immunityRole of Staphylococcus aureus Formate Metabolism during Prosthetic Joint Infection.PMC9670962P30 CA036727P30 GM110768P20 GM103427P01 AI083211R01 AI125588West SCAli HHeim CEThomas VCKielian TBertrand BPChaudhari SSfalseRole of Staphylococcus aureus Formate Metabolism during Prosthetic Joint Infection.Biofilms are bacterial communities characterized by antibiotic tolerance. Staphylococcus aureus is a leading cause of biofilm infections on medical devices, including prosthetic joints, which represent a significant health care burden. The major leukocyte infiltrate associated with S. aureus prosthetic joint infection (PJI) is granulocytic myeloid-derived suppressor cells (G-MDSCs), which produce IL-10 to promote biofilm persistence by inhibiting monocyte and macrophage proinflammatory activity. To determine how S. aureus biofilm responds to G-MDSCs and macrophages, biofilms were cocultured with either leukocyte population followed by RNA sequencing. Several genes involved in fermentative pathways were significantly upregulated in S. aureus biofilm following G-MDSC coculture, including for2022-01-01T00:00:00Z2022 Nov2026-06-13T06:16:14.778Z2025-04-06T14:10:09.067ZS-EPMC96709623628652510.1128/iai.00428-22