biostudies-literatureUnknown9Chen ZNatural Science Foundation of Heilongjiang ProvinceThe present study aimed to search for functional mutations within the promoter of porcine <i>STAT3</i> and to provide causative genetic variants associated with piglet diarrhea. We firstly confirmed that <i>STAT3</i> expressed higher in the small intestine than in the spleen, stomach and large intestine of SPF piglets, respectively (<i>P</i> < 0.05). Then, 10 genetic variations in the porcine STAT3 promoter region was identified by direct sequencing. Among them, three mutations SNP1: g.-870 G>A, SNP2: g.-584 A>C and a 6-bp Indel in the promoter region that displayed significant differential transcriptional activities were identified. Association analyses showed that SNP1: g.-870 G>A was significantly associated with piglet diarrhea (<i>P</i> < 0.05) and the GG animals had lower diarrhea scFrontiers in veterinary science1034187https://www.ebi.ac.uk/biostudies/studies/S-EPMC9672087biostudies-literatureA functional mutation associated with piglet diarrhea partially by regulating the transcription of porcine <i>STAT3</i>.PMC9672087Yang XNiu BLiu LChen ZYao DDi SCai JGuo DKou MWang XSun YfalseA functional mutation associated with piglet diarrhea partially by regulating the transcription of porcine <i>STAT3</i>.The present study aimed to search for functional mutations within the promoter of porcine <i>STAT3</i> and to provide causative genetic variants associated with piglet diarrhea. We firstly confirmed that <i>STAT3</i> expressed higher in the small intestine than in the spleen, stomach and large intestine of SPF piglets, respectively (<i>P</i> < 0.05). Then, 10 genetic variations in the porcine STAT3 promoter region was identified by direct sequencing. Among them, three mutations SNP1: g.-870 G>A, SNP2: g.-584 A>C and a 6-bp Indel in the promoter region that displayed significant differential transcriptional activities were identified. Association analyses showed that SNP1: g.-870 G>A was significantly associated with piglet diarrhea (<i>P</i> < 0.05) and the GG animals had lower diarrhea sc2022-01-01T00:00:00Z20222025-04-26T22:32:04.508Z2025-04-06T17:16:04.06ZS-EPMC96720873640608910.3389/fvets.2022.1034187