{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Mazgaoker S"],"funding":["US-Israel Binational Science Foundation","Israel Science Foundation"],"pagination":["e202213153"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9674091"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["155(1)"],"pubmed_abstract":["Human-induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) have been used to screen and characterize drugs and to reveal mechanisms underlying cardiac diseases. However, before hiPSC-CMs can be used as a reliable experimental model, the physiological mechanisms underlying their normal function should be further explored. Accordingly, a major feature of hiPSC-CMs is automaticity, which is regulated by both Ca2+ and membrane clocks. To investigate the mechanisms coupling these clocks, we tested three hypotheses: (1) normal automaticity of spontaneously beating hiPSC-CMs is regulated by local Ca2+ releases (LCRs) and cAMP/PKA-dependent coupling of Ca2+ clock to M clock; (2) the LCR period indicates the level of crosstalk within the coupled-clock system; and (3) perturbing the acti"],"journal":["The Journal of general physiology"],"pubmed_title":["cAMP-PKA signaling modulates the automaticity of human iPSC-derived cardiomyocytes."],"pmcid":["PMC9674091"],"funding_grant_id":["ISF 824/19"],"pubmed_authors":["Mazgaoker S","Weiser-Bitoun I","Brosh I","Yaniv Y","Binah O"],"additional_accession":[]},"is_claimable":false,"name":"cAMP-PKA signaling modulates the automaticity of human iPSC-derived cardiomyocytes.","description":"Human-induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) have been used to screen and characterize drugs and to reveal mechanisms underlying cardiac diseases. However, before hiPSC-CMs can be used as a reliable experimental model, the physiological mechanisms underlying their normal function should be further explored. Accordingly, a major feature of hiPSC-CMs is automaticity, which is regulated by both Ca2+ and membrane clocks. To investigate the mechanisms coupling these clocks, we tested three hypotheses: (1) normal automaticity of spontaneously beating hiPSC-CMs is regulated by local Ca2+ releases (LCRs) and cAMP/PKA-dependent coupling of Ca2+ clock to M clock; (2) the LCR period indicates the level of crosstalk within the coupled-clock system; and (3) perturbing the acti","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Jan","modification":"2025-04-22T18:28:59.371Z","creation":"2025-04-06T02:27:15.993Z"},"accession":"S-EPMC9674091","cross_references":{"pubmed":["36383232"],"doi":["10.1085/jgp.202213153"]}}