{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Matsumura T"],"funding":["MOH | National Medical Research Council","MEXT | Japan Society for the Promotion of Science","NOVARTIS Foundation (Japan) for the Promotion of Science (NOVARTIS Foundation","Daiwa Securities Health Foundation"],"pagination":["7064"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9674656"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["13(1)"],"pubmed_abstract":["The transcription factor MYB is a crucial regulator of hematopoietic stem and progenitor cells. However, the nature of lineage-specific enhancer usage of the Myb gene is largely unknown. We identify the Myb -68 enhancer, a regulatory element which marks basophils and mast cells. Using the Myb -68 enhancer activity, we show a population of granulocyte-macrophage progenitors with higher potential to differentiate into basophils and mast cells. Single cell RNA-seq demonstrates the differentiation trajectory is continuous from progenitors to mature basophils in vivo, characterizes bone marrow cells with a gene signature of mast cells, and identifies LILRB4 as a surface marker of basophil maturation. Together, our study leads to a better understanding of how MYB expression is regulated in a lineage-associated manner, and also shows how a combination of lineage-related reporter mice and single-cell transcriptomics can overcome the rarity of target cells and enhance our understanding of gene expression programs that control cell differentiation in vivo."],"journal":["Nature communications"],"pubmed_title":["A Myb enhancer-guided analysis of basophil and mast cell differentiation."],"pmcid":["PMC9674656"],"funding_grant_id":["NMRC/CNIG/1155/2016","JP21K19514"],"pubmed_authors":["Fukuda M","Deng J","Rethnam M","Matsumura T","Yang C","Karasawa T","Yokomori R","Suda T","Osato M","Tan TK","Totani H","Gunji Y","Sanda T","Takahashi M","Kario K"],"additional_accession":[]},"is_claimable":false,"name":"A Myb enhancer-guided analysis of basophil and mast cell differentiation.","description":"The transcription factor MYB is a crucial regulator of hematopoietic stem and progenitor cells. However, the nature of lineage-specific enhancer usage of the Myb gene is largely unknown. We identify the Myb -68 enhancer, a regulatory element which marks basophils and mast cells. Using the Myb -68 enhancer activity, we show a population of granulocyte-macrophage progenitors with higher potential to differentiate into basophils and mast cells. Single cell RNA-seq demonstrates the differentiation trajectory is continuous from progenitors to mature basophils in vivo, characterizes bone marrow cells with a gene signature of mast cells, and identifies LILRB4 as a surface marker of basophil maturation. Together, our study leads to a better understanding of how MYB expression is regulated in a lineage-associated manner, and also shows how a combination of lineage-related reporter mice and single-cell transcriptomics can overcome the rarity of target cells and enhance our understanding of gene expression programs that control cell differentiation in vivo.","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Nov","modification":"2026-05-27T11:56:38.379Z","creation":"2025-04-19T06:03:31.992Z"},"accession":"S-EPMC9674656","cross_references":{"pubmed":["36400777"],"doi":["10.1038/s41467-022-34906-1"]}}