biostudies-literatureUnknown13Zhu L<i>Arnebiae</i> Radix (dried root of <i>Arnebia euchroma</i> (Royle) Johnst.) is a traditional Chinese medicine (TCM) used to treat macular eruptions, measles, sore throat, carbuncles, burns, skin ulcers, and inflammations. The <i>Arnebiae</i> Radix extract can exert anti-breast cancer effects through various mechanisms of action. This study aimed to rapidly screen potential estrogen receptor (estrogen receptor α and estrogen receptor β) ligands from the <i>Arnebiae</i> Radix extract. In this study, an analytical method based on affinity ultrafiltration coupled with UHPLC-Q-Exactive Orbitrap mass spectrometry was established for rapidly screening and identifying estrogen receptor ligands. Then, bindings of the components to the active site of estrogen receptor (estrogen receptor α and estrFrontiers in plant science1012553https://www.ebi.ac.uk/biostudies/studies/S-EPMC9676231biostudies-literatureScreening and characterization estrogen receptor ligands from <i>Arnebia euchroma</i> (Royle) Johnst. <i>via</i> affinity ultrafiltration LC-MS and molecular docking.PMC9676231Wang ZMLi SNZhu LLiu MJZhang SLE SMa SJLi KLCai WfalseScreening and characterization estrogen receptor ligands from <i>Arnebia euchroma</i> (Royle) Johnst. <i>via</i> affinity ultrafiltration LC-MS and molecular docking.<i>Arnebiae</i> Radix (dried root of <i>Arnebia euchroma</i> (Royle) Johnst.) is a traditional Chinese medicine (TCM) used to treat macular eruptions, measles, sore throat, carbuncles, burns, skin ulcers, and inflammations. The <i>Arnebiae</i> Radix extract can exert anti-breast cancer effects through various mechanisms of action. This study aimed to rapidly screen potential estrogen receptor (estrogen receptor α and estrogen receptor β) ligands from the <i>Arnebiae</i> Radix extract. In this study, an analytical method based on affinity ultrafiltration coupled with UHPLC-Q-Exactive Orbitrap mass spectrometry was established for rapidly screening and identifying estrogen receptor ligands. Then, bindings of the components to the active site of estrogen receptor (estrogen receptor α and estr2022-01-01T00:00:00Z20222025-04-18T20:33:32.59Z2025-04-07T08:29:38.479ZS-EPMC96762313642002910.3389/fpls.2022.1012553