{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["13"],"submitter":["Joshi I"],"pubmed_abstract":["One of the major obligate plant parasites causing massive economic crop losses belongs to the class of root-knot nematodes (RKNs). Targeting of major nematode parasitism genes <i>via</i> Host Delivered-RNAi (HD-RNAi) to confer silencing is established as one of the most effective approaches to curb nematode infection. Utilizing nematode-responsive root-specific (NRRS) promoters to design a dsRNA molecule targeting approach to hamper nematode parasitism. Here, a previously validated peroxidase gall specific promoter, p<i>At2g18140</i>, from <i>Arabidopsis</i> was employed to express the dsRNA construct of the nematode effector gene <i>Mi-msp2</i> from <i>Meloidogyne incognita</i>. <i>Arabidopsis</i> RNAi lines of <i>CaMV35S::Mi-msp2-RNAi</i> and p<i>At2g18140::Mi-msp2-RNAi</i> were compared"],"journal":["Frontiers in plant science"],"pagination":["1007322"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9679145"],"repository":["biostudies-literature"],"pubmed_title":["Gall-specific promoter, an alternative to the constitutive <i>CaMV35S</i> promoter, drives host-derived RNA interference targeting <i>Mi-msp2</i> gene to confer effective nematode resistance."],"pmcid":["PMC9679145"],"pubmed_authors":["Sirohi A","Jain PK","Joshi I","Chaudhury A","Bhattacharya R","Kohli D","Kumar A"],"additional_accession":[]},"is_claimable":false,"name":"Gall-specific promoter, an alternative to the constitutive <i>CaMV35S</i> promoter, drives host-derived RNA interference targeting <i>Mi-msp2</i> gene to confer effective nematode resistance.","description":"One of the major obligate plant parasites causing massive economic crop losses belongs to the class of root-knot nematodes (RKNs). Targeting of major nematode parasitism genes <i>via</i> Host Delivered-RNAi (HD-RNAi) to confer silencing is established as one of the most effective approaches to curb nematode infection. Utilizing nematode-responsive root-specific (NRRS) promoters to design a dsRNA molecule targeting approach to hamper nematode parasitism. Here, a previously validated peroxidase gall specific promoter, p<i>At2g18140</i>, from <i>Arabidopsis</i> was employed to express the dsRNA construct of the nematode effector gene <i>Mi-msp2</i> from <i>Meloidogyne incognita</i>. <i>Arabidopsis</i> RNAi lines of <i>CaMV35S::Mi-msp2-RNAi</i> and p<i>At2g18140::Mi-msp2-RNAi</i> were compared","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022","modification":"2025-07-13T03:04:03.594Z","creation":"2025-04-19T16:11:46.522Z"},"accession":"S-EPMC9679145","cross_references":{"pubmed":["36426141"],"doi":["10.3389/fpls.2022.1007322"]}}