biostudies-literatureUnknown13Joshi IOne of the major obligate plant parasites causing massive economic crop losses belongs to the class of root-knot nematodes (RKNs). Targeting of major nematode parasitism genes <i>via</i> Host Delivered-RNAi (HD-RNAi) to confer silencing is established as one of the most effective approaches to curb nematode infection. Utilizing nematode-responsive root-specific (NRRS) promoters to design a dsRNA molecule targeting approach to hamper nematode parasitism. Here, a previously validated peroxidase gall specific promoter, p<i>At2g18140</i>, from <i>Arabidopsis</i> was employed to express the dsRNA construct of the nematode effector gene <i>Mi-msp2</i> from <i>Meloidogyne incognita</i>. <i>Arabidopsis</i> RNAi lines of <i>CaMV35S::Mi-msp2-RNAi</i> and p<i>At2g18140::Mi-msp2-RNAi</i> were comparedFrontiers in plant science1007322https://www.ebi.ac.uk/biostudies/studies/S-EPMC9679145biostudies-literatureGall-specific promoter, an alternative to the constitutive <i>CaMV35S</i> promoter, drives host-derived RNA interference targeting <i>Mi-msp2</i> gene to confer effective nematode resistance.PMC9679145Sirohi AJain PKJoshi IChaudhury ABhattacharya RKohli DKumar AfalseGall-specific promoter, an alternative to the constitutive <i>CaMV35S</i> promoter, drives host-derived RNA interference targeting <i>Mi-msp2</i> gene to confer effective nematode resistance.One of the major obligate plant parasites causing massive economic crop losses belongs to the class of root-knot nematodes (RKNs). Targeting of major nematode parasitism genes <i>via</i> Host Delivered-RNAi (HD-RNAi) to confer silencing is established as one of the most effective approaches to curb nematode infection. Utilizing nematode-responsive root-specific (NRRS) promoters to design a dsRNA molecule targeting approach to hamper nematode parasitism. Here, a previously validated peroxidase gall specific promoter, p<i>At2g18140</i>, from <i>Arabidopsis</i> was employed to express the dsRNA construct of the nematode effector gene <i>Mi-msp2</i> from <i>Meloidogyne incognita</i>. <i>Arabidopsis</i> RNAi lines of <i>CaMV35S::Mi-msp2-RNAi</i> and p<i>At2g18140::Mi-msp2-RNAi</i> were compared2022-01-01T00:00:00Z20222025-07-13T03:04:03.594Z2025-04-19T16:11:46.522ZS-EPMC96791453642614110.3389/fpls.2022.1007322