biostudies-literatureSrivastava SNCI NIH HHSNational Institutes of HealthNIGMS NIH HHS1116-1136https://www.ebi.ac.uk/biostudies/studies/S-EPMC9680117biostudies-literatureUnknown32(12)Glycans that are abundantly displayed on vertebrate cell surface and secreted molecules are often capped with terminal sialic acids (Sias). These diverse 9-carbon-backbone monosaccharides are involved in numerous intrinsic biological processes. They also interact with commensals and pathogens, while undergoing dynamic changes in time and space, often influenced by environmental conditions. However, most of this sialoglycan complexity and variation remains poorly characterized by conventional techniques, which often tend to destroy or overlook crucial aspects of Sia diversity and/or fail to elucidate native structures in biological systems, i.e. in the intact sialome. To date, in situ detection and analysis of sialoglycans has largely relied on the use of plant lectins, sialidases, or antibGlycobiologyDevelopment and applications of sialoglycan-recognizing probes (SGRPs) with defined specificities: exploring the dynamic mammalian sialoglycome.PMC9680117R01 GM032373R01GM32373U01CA199792U01 CA199792Bensing BAVarki NSecrest PDiaz SYu HVerhagen ASrivastava SVarki AKhan NSullam PChen XParrish CRSasmal AWasik BRKhedri ZfalseDevelopment and applications of sialoglycan-recognizing probes (SGRPs) with defined specificities: exploring the dynamic mammalian sialoglycome.Glycans that are abundantly displayed on vertebrate cell surface and secreted molecules are often capped with terminal sialic acids (Sias). These diverse 9-carbon-backbone monosaccharides are involved in numerous intrinsic biological processes. They also interact with commensals and pathogens, while undergoing dynamic changes in time and space, often influenced by environmental conditions. However, most of this sialoglycan complexity and variation remains poorly characterized by conventional techniques, which often tend to destroy or overlook crucial aspects of Sia diversity and/or fail to elucidate native structures in biological systems, i.e. in the intact sialome. To date, in situ detection and analysis of sialoglycans has largely relied on the use of plant lectins, sialidases, or antib2022-01-01T00:00:00Z2022 Nov2026-05-28T02:11:16.465Z2024-12-04T05:26:56.574ZS-EPMC96801173592609010.1093/glycob/cwac050