biostudies-literatureTemirel MMarie Skłodowska-Curie Individual FellowshipRoyal Academy Newton-Katip Çelebi Transforming Systems Through Partnership awardTubitak 2232 International Fellowship for the Outstanding Researchers AwardAlexander von Humboldt Research Fellowship for Experienced Researchers225https://www.ebi.ac.uk/biostudies/studies/S-EPMC9680455biostudies-literatureUnknown13(4)Extrusion-based 3D bioprinting is a promising technique for fabricating multi-layered, complex biostructures, as it enables multi-material dispersion of bioinks with a straightforward procedure (particularly for users with limited additive manufacturing skills). Nonetheless, this method faces challenges in retaining the shape fidelity of the 3D-bioprinted structure, i.e., the collapse of filament (bioink) due to gravity and/or spreading of the bioink owing to the low viscosity, ultimately complicating the fabrication of multi-layered designs that can maintain the desired pore structure. While low viscosity is required to ensure a continuous flow of material (without clogging), a bioink should be viscous enough to retain its shape post-printing, highlighting the importance of bioink propertJournal of functional biomaterialsShape Fidelity Evaluation of Alginate-Based Hydrogels through Extrusion-Based Bioprinting.PMC9680455118C391120N019101003361Tasoglu STemirel MDabbagh SRfalseShape Fidelity Evaluation of Alginate-Based Hydrogels through Extrusion-Based Bioprinting.Extrusion-based 3D bioprinting is a promising technique for fabricating multi-layered, complex biostructures, as it enables multi-material dispersion of bioinks with a straightforward procedure (particularly for users with limited additive manufacturing skills). Nonetheless, this method faces challenges in retaining the shape fidelity of the 3D-bioprinted structure, i.e., the collapse of filament (bioink) due to gravity and/or spreading of the bioink owing to the low viscosity, ultimately complicating the fabrication of multi-layered designs that can maintain the desired pore structure. While low viscosity is required to ensure a continuous flow of material (without clogging), a bioink should be viscous enough to retain its shape post-printing, highlighting the importance of bioink propert2022-01-01T00:00:00Z2022 Nov2025-04-04T22:14:46.422Z2025-04-04T22:14:46.422ZS-EPMC96804553641286610.3390/jfb13040225