{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Saito AN"],"funding":["Takeda Science Foundation","Japan Society for the Promotion of Science"],"pagination":["1720-1728"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9680855"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["63(11)"],"pubmed_abstract":["The circadian clock, an internal time-keeping system with a period of about 24 h, coordinates many physiological processes with the day-night cycle. We previously demonstrated that BML-259 [N-(5-isopropyl-2-thiazolyl) phenylacetamide], a small molecule with mammal CYCLIN DEPENDENT KINASE 5 (CDK5)/CDK2 inhibition activity, lengthens Arabidopsis thaliana (Arabidopsis) circadian clock periods. BML-259 inhibits Arabidopsis CDKC kinase, which phosphorylates RNA polymerase II in the general transcriptional machinery. To accelerate our understanding of the inhibitory mechanism of BML-259 on CDKC, we performed structure-function studies of BML-259 using circadian period-lengthening activity as an estimation of CDKC inhibitor activity in vivo. The presence of a thiazole ring is essential for period"],"journal":["Plant & cell physiology"],"pubmed_title":["Structure-Function Study of a Novel Inhibitor of Cyclin-Dependent Kinase C in Arabidopsis."],"pmcid":["PMC9680855"],"funding_grant_id":["18H04428","20H05411","20K21272","21H05656"],"pubmed_authors":["Matsuo H","Fujimoto KJ","Maeda AE","Nakamichi N","Nishina M","Yanai T","Ota E","Notaguchi M","Saito AN","Takahara TT","Kinoshita T","Yamaguchi J","Ono A","Shiratake K"],"additional_accession":[]},"is_claimable":false,"name":"Structure-Function Study of a Novel Inhibitor of Cyclin-Dependent Kinase C in Arabidopsis.","description":"The circadian clock, an internal time-keeping system with a period of about 24 h, coordinates many physiological processes with the day-night cycle. We previously demonstrated that BML-259 [N-(5-isopropyl-2-thiazolyl) phenylacetamide], a small molecule with mammal CYCLIN DEPENDENT KINASE 5 (CDK5)/CDK2 inhibition activity, lengthens Arabidopsis thaliana (Arabidopsis) circadian clock periods. BML-259 inhibits Arabidopsis CDKC kinase, which phosphorylates RNA polymerase II in the general transcriptional machinery. To accelerate our understanding of the inhibitory mechanism of BML-259 on CDKC, we performed structure-function studies of BML-259 using circadian period-lengthening activity as an estimation of CDKC inhibitor activity in vivo. The presence of a thiazole ring is essential for period","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Nov","modification":"2026-04-08T11:06:32.928Z","creation":"2025-04-19T06:03:29.961Z"},"accession":"S-EPMC9680855","cross_references":{"pubmed":["36043692"],"doi":["10.1093/pcp/pcac127"]}}