biostudies-literatureGarcia FJNICHD NIH HHSNIA NIH HHSNIMH NIH HHSNHGRI NIH HHSNINDS NIH HHSNIH HHS893-899https://www.ebi.ac.uk/biostudies/studies/S-EPMC9680899biostudies-literatureUnknown603(7903)Despite the importance of the cerebrovasculature in maintaining normal brain physiology and in understanding neurodegeneration and drug delivery to the central nervous system¹, human cerebrovascular cells remain poorly characterized owing to their sparsity and dispersion. Here we perform single-cell characterization of the human cerebrovasculature using both ex vivo fresh tissue experimental enrichment and post mortem in silico sorting of human cortical tissue samples. We capture 16,681 cerebrovascular nuclei across 11 subtypes, including endothelial cells, mural cells and three distinct subtypes of perivascular fibroblast along the vasculature. We uncover human-specific expression patterns along the arteriovenous axis and determine previously uncharacterized cell-type-specific NatureSingle-cell dissection of the human brain vasculature.PMC9680899R01 AG015819U01 AG061356R56 AG067151R01 AG15819P30 AG010161R01 AG067151R01 AG17917U01 AG61356R01 AG058002P50 HD105351P30 AG10161U01 NS110453P30 AG072975RF1 AG054012R01 MH109978U01 MH119509R01 AG062335UH3 NS115064RF1 AG062377R01 AG017917R01 HG008155U54 HD090255UG3 NS115064Lee HNg APKellis MGalani KMathys HSun NBennett DAGarcia FJZhou BJiang XMantero JTsai LHSahin MGodlewski BHeiman MfalseSingle-cell dissection of the human brain vasculature.Despite the importance of the cerebrovasculature in maintaining normal brain physiology and in understanding neurodegeneration and drug delivery to the central nervous system¹, human cerebrovascular cells remain poorly characterized owing to their sparsity and dispersion. Here we perform single-cell characterization of the human cerebrovasculature using both ex vivo fresh tissue experimental enrichment and post mortem in silico sorting of human cortical tissue samples. We capture 16,681 cerebrovascular nuclei across 11 subtypes, including endothelial cells, mural cells and three distinct subtypes of perivascular fibroblast along the vasculature. We uncover human-specific expression patterns along the arteriovenous axis and determine previously uncharacterized cell-type-specific 2022-01-01T00:00:00Z2022 Mar2026-05-08T03:08:17.306Z2025-02-19T04:20:08.144ZS-EPMC96808993515837110.1038/s41586-022-04521-7