biostudies-literatureWang FNatural Science Foundation of Zhejiang Province1630260https://www.ebi.ac.uk/biostudies/studies/S-EPMC9681548biostudies-literatureUnknown2022This study aimed to investigate the molecular mechanisms of microRNA-146a (miR-146a) on gingival mesenchymal stem cells (MSCs). Gingival MSCs were isolated from the gingiva tissues of patients with periodontal disease to reveal the function of miR-146a in regulating osteoblast differentiation. miR-146a inhibits osteoblast differentiation by inhibiting phosphorylated cyclic-AMP response binding (CREB) protein translocation into the nucleus and ultimately attenuating runt-related transcription factor 2 (Runx2) expression. Furthermore, silencing miR-146a promotes the proliferation of gingival MSCs. Of note, targeted inhibition of miR-146a also inhibited LPS-induced inflammatory response and promoted the proliferation of gingival MSCs via CREB/Runx2 axis. MiR-146a is a key negative regulator oEvidence-based complementary and alternative medicine : eCAMStudy on the Mechanism of miR-146a in Gingival Mesenchymal Stem Cells.PMC96815482020386978LY18C100001Li XJing ZWei TJiang HWang FfalseStudy on the Mechanism of miR-146a in Gingival Mesenchymal Stem Cells.This study aimed to investigate the molecular mechanisms of microRNA-146a (miR-146a) on gingival mesenchymal stem cells (MSCs). Gingival MSCs were isolated from the gingiva tissues of patients with periodontal disease to reveal the function of miR-146a in regulating osteoblast differentiation. miR-146a inhibits osteoblast differentiation by inhibiting phosphorylated cyclic-AMP response binding (CREB) protein translocation into the nucleus and ultimately attenuating runt-related transcription factor 2 (Runx2) expression. Furthermore, silencing miR-146a promotes the proliferation of gingival MSCs. Of note, targeted inhibition of miR-146a also inhibited LPS-induced inflammatory response and promoted the proliferation of gingival MSCs via CREB/Runx2 axis. MiR-146a is a key negative regulator o2022-01-01T00:00:00Z20222025-04-25T20:32:51.408Z2025-04-06T08:23:45.232ZS-EPMC96815483642525910.1155/2022/1630260