biostudies-literatureUnknown2(1)Doan TNK<h4>Background</h4>Adeno-associated virus (AAV) vectors are stored and shipped frozen which poses logistic and economic barriers for global access to these therapeutics. To address this issue, we developed a method to stabilize AAV serotype 9 (AAV9) in a film matrix that can be stored at ambient temperature and administered by systemic injection.<h4>Methods</h4>AAV9 expressing the luciferase transgene was mixed with formulations, poured into molds and films dried under aseptic conditions. Films were packaged in individual particle-free bags with foil overlays and stored at various temperatures under controlled humidity. Recovery of AAV9 from films was determined by serial dilution of rehydrated film in media and infection of HeLa RC32 cells. Luciferase expression was compared to that of fiCommunications medicine148https://www.ebi.ac.uk/biostudies/studies/S-EPMC9681776biostudies-literatureThermostability and in vivo performance of AAV9 in a film matrix.PMC9681776Le MDMote ACelentano LTretiakova ABajrovic IKrause CBalyan HGDoan TNKSvancarek AJude Samulski RCroyle MAfalseThermostability and in vivo performance of AAV9 in a film matrix.<h4>Background</h4>Adeno-associated virus (AAV) vectors are stored and shipped frozen which poses logistic and economic barriers for global access to these therapeutics. To address this issue, we developed a method to stabilize AAV serotype 9 (AAV9) in a film matrix that can be stored at ambient temperature and administered by systemic injection.<h4>Methods</h4>AAV9 expressing the luciferase transgene was mixed with formulations, poured into molds and films dried under aseptic conditions. Films were packaged in individual particle-free bags with foil overlays and stored at various temperatures under controlled humidity. Recovery of AAV9 from films was determined by serial dilution of rehydrated film in media and infection of HeLa RC32 cells. Luciferase expression was compared to that of fi2022-01-01T00:00:00Z2022 Nov2026-06-03T10:38:07.27Z2025-02-19T03:57:32.727ZS-EPMC96817763641477310.1038/s43856-022-00212-6