{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Mirabelli C"],"funding":["EC | Horizon Europe | Excellent Science | HORIZON EUROPE Marie Sklodowska-Curie Actions","HHS | NIH | OSC | Common Fund (NIH Common Fund)","UM | Michigan Institute for Clinical and Health Research","UM | Michigan Institute for Clinical and Health Research (MICHR)","NIEHS NIH HHS","HHS | NIH | OSC | Common Fund","EC | Horizon Europe | HORIZON EUROPE Innovative Europe"],"pagination":["e0085522"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9683019"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["96(22)"],"pubmed_abstract":["Human norovirus (HNoV) accounts for one-fifth of all acute viral gastroenteritis worldwide and an economic burden of ~$60 billion globally. The lack of treatment options against HNoV is in part due to the lack of cultivation systems. Recently, a model of infection in biopsy-derived human intestinal enteroids (HIE) has been described: 3D-HIE are first dispersed in 2D-monolayers and differentiated prior to infection, resulting in a labor-intensive, time-consuming procedure. Here, we present an alternative protocol for HNoV infection of 3D-HIE. We found that 3D-HIE differentiated as efficiently as 2D-monolayers. In addition, immunofluorescence-based quantification of UEA-1, a lectin that stains the villus brush border, revealed that ~80% of differentiated 3D-HIE spontaneously undergo polarity"],"journal":["Journal of virology"],"pubmed_title":["Human Norovirus Efficiently Replicates in Differentiated 3D-Human Intestinal Enteroids."],"pmcid":["PMC9683019"],"funding_grant_id":["812673","R01 ES028802","P30 ES017885","P30ES017885","841247","UL1TR002240","P30DK034933"],"pubmed_authors":["Sexton JZ","Wobus CE","Santos-Ferreira N","Rocha-Pereira J","Gillilland MG","Neyts J","Colacino JA","Cieza RJ","Mirabelli C","Taube S"],"additional_accession":[]},"is_claimable":false,"name":"Human Norovirus Efficiently Replicates in Differentiated 3D-Human Intestinal Enteroids.","description":"Human norovirus (HNoV) accounts for one-fifth of all acute viral gastroenteritis worldwide and an economic burden of ~$60 billion globally. The lack of treatment options against HNoV is in part due to the lack of cultivation systems. Recently, a model of infection in biopsy-derived human intestinal enteroids (HIE) has been described: 3D-HIE are first dispersed in 2D-monolayers and differentiated prior to infection, resulting in a labor-intensive, time-consuming procedure. Here, we present an alternative protocol for HNoV infection of 3D-HIE. We found that 3D-HIE differentiated as efficiently as 2D-monolayers. In addition, immunofluorescence-based quantification of UEA-1, a lectin that stains the villus brush border, revealed that ~80% of differentiated 3D-HIE spontaneously undergo polarity","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Nov","modification":"2026-06-14T03:31:38.457Z","creation":"2025-02-19T03:25:40.852Z"},"accession":"S-EPMC9683019","cross_references":{"pubmed":["36342297"],"doi":["10.1128/jvi.00855-22"]}}