{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Tayyab M"],"funding":["DARPA Cooperative Agreement","National Science Foundation"],"pagination":["20119"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9684124"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["12(1)"],"pubmed_abstract":["Proteins are useful biomarkers for a wide range of applications such as cancer detection, discovery of vaccines, and determining exposure to viruses and pathogens. Here, we present a low-noise front-end analog circuit interface towards development of a portable readout system for the label-free sensing of proteins using Nanowell array impedance sensing with a form factor of approximately 35cm<sup>2</sup>. The electronic interface consists of a low-noise lock-in amplifier enabling reliable detection of changes in impedance as low as 0.1% and thus detection of proteins down to the picoMolar level. The sensitivity of our system is comparable to that of a commercial bench-top impedance spectroscope when using the same sensors. The aim of this work is to demonstrate the potential of using impedance sensing as a portable, low-cost, and reliable method of detecting proteins, thus inching us closer to a Point-of-Care (POC) personalized health monitoring system. We have demonstrated the utility of our system to detect antibodies at various concentrations and protein (45 pM IL-6) in PBS, however, our system has the capability to be used for assaying various biomarkers including proteins, cytokines, virus molecules and antibodies in a portable setting."],"journal":["Scientific reports"],"pubmed_title":["A portable analog front-end system for label-free sensing of proteins using nanowell array impedance sensors."],"pmcid":["PMC9684124"],"funding_grant_id":["IDBR Award No. 1846740,CAREER Award No. 1556253, and ECCS-CCSS Award No. 1711165.","HR0011-16-2-0026"],"pubmed_authors":["Raji H","Sami MA","Mahmoodi SR","Javanmard M","Tayyab M","Lin Z","Meng Z","Xie P"],"additional_accession":[]},"is_claimable":false,"name":"A portable analog front-end system for label-free sensing of proteins using nanowell array impedance sensors.","description":"Proteins are useful biomarkers for a wide range of applications such as cancer detection, discovery of vaccines, and determining exposure to viruses and pathogens. Here, we present a low-noise front-end analog circuit interface towards development of a portable readout system for the label-free sensing of proteins using Nanowell array impedance sensing with a form factor of approximately 35cm<sup>2</sup>. The electronic interface consists of a low-noise lock-in amplifier enabling reliable detection of changes in impedance as low as 0.1% and thus detection of proteins down to the picoMolar level. The sensitivity of our system is comparable to that of a commercial bench-top impedance spectroscope when using the same sensors. The aim of this work is to demonstrate the potential of using impedance sensing as a portable, low-cost, and reliable method of detecting proteins, thus inching us closer to a Point-of-Care (POC) personalized health monitoring system. We have demonstrated the utility of our system to detect antibodies at various concentrations and protein (45 pM IL-6) in PBS, however, our system has the capability to be used for assaying various biomarkers including proteins, cytokines, virus molecules and antibodies in a portable setting.","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Nov","modification":"2025-04-27T03:03:28.989Z","creation":"2025-04-06T18:39:58.76Z"},"accession":"S-EPMC9684124","cross_references":{"pubmed":["36418852"],"doi":["10.1038/s41598-022-23286-7"]}}