biostudies-literatureKoren JEuropean Structural and Investment FundsSlovak Research and Development AgencyEuropean Regional Development Fund1538https://www.ebi.ac.uk/biostudies/studies/S-EPMC9686475biostudies-literatureUnknown11(11)Carbapenem-resistant (CR) <i>Klebsiella pneumoniae</i> represents an urgent worldwide threat. We focused on CR <i>K. pneumoniae</i> in selected facilities of the University Hospital Bratislava (UHB) to investigate sequence types (STs), clonal relatedness, and antimicrobial resistance. Suspected carbapenem-nonsusceptible <i>K. pneumoniae</i> strains were obtained from hospitalized patients. Further examination included carbapenemase confirmation, cgMLST, and quantitative susceptibility testing. Of the total 41 CR <i>K. pneumoniae</i> strains, 26 (63.4%) were determined as ST11 in hospital No. 1; of these ST11, 22 (84.6%) were found in the first internal clinic. Six (14.6%) ST258 and three (7.3%) ST584 occurred in hospital No. 2; the most, i.e., four (66.7%), ST258 were detected in the geriatric clinic. In hospital No. 3, we found two (4.8%) ST584 and one (2.4%) ST258. Of the ST11 set, 24 (92.3%) produced NDM-1. Furthermore, seven (87.5) ST258 and five (100%) ST584 strains generated KPC-2. Antimicrobial resistance was as follows: ertapenem 97.6%, meropenem 63.4%, tigecycline 7.3%, eravacycline 7.3%, and colistin 2.5%. We revealed a presumably epidemiological association of ST11 with transmission, particularly in the first internal clinic of hospital No. 1, while ST258 and ST584 were related to interhospital dissemination between medical facilities No. 2 and No. 3. It is essential to prevent the circulation of these pathogens within and between healthcare facilities.Antibiotics (Basel, Switzerland)Next-Generation Sequencing of Carbapenem-Resistant <i>Klebsiella pneumoniae</i> Strains Isolated from Patients Hospitalized in the University Hospital Facilities.PMC9686475SRDA-20-0413ITMS: 313011T431NFP313010V344Maliar TKoren JLiptakova ADrahovska HHubenakova ZAndrezal MfalseNext-Generation Sequencing of Carbapenem-Resistant <i>Klebsiella pneumoniae</i> Strains Isolated from Patients Hospitalized in the University Hospital Facilities.Carbapenem-resistant (CR) <i>Klebsiella pneumoniae</i> represents an urgent worldwide threat. We focused on CR <i>K. pneumoniae</i> in selected facilities of the University Hospital Bratislava (UHB) to investigate sequence types (STs), clonal relatedness, and antimicrobial resistance. Suspected carbapenem-nonsusceptible <i>K. pneumoniae</i> strains were obtained from hospitalized patients. Further examination included carbapenemase confirmation, cgMLST, and quantitative susceptibility testing. Of the total 41 CR <i>K. pneumoniae</i> strains, 26 (63.4%) were determined as ST11 in hospital No. 1; of these ST11, 22 (84.6%) were found in the first internal clinic. Six (14.6%) ST258 and three (7.3%) ST584 occurred in hospital No. 2; the most, i.e., four (66.7%), ST258 were detected in the geriatric clinic. In hospital No. 3, we found two (4.8%) ST584 and one (2.4%) ST258. Of the ST11 set, 24 (92.3%) produced NDM-1. Furthermore, seven (87.5) ST258 and five (100%) ST584 strains generated KPC-2. Antimicrobial resistance was as follows: ertapenem 97.6%, meropenem 63.4%, tigecycline 7.3%, eravacycline 7.3%, and colistin 2.5%. We revealed a presumably epidemiological association of ST11 with transmission, particularly in the first internal clinic of hospital No. 1, while ST258 and ST584 were related to interhospital dissemination between medical facilities No. 2 and No. 3. It is essential to prevent the circulation of these pathogens within and between healthcare facilities.2022-01-01T00:00:00Z2022 Nov2025-04-25T20:58:36.359Z2025-04-06T08:38:50.918ZS-EPMC96864753635819310.3390/antibiotics11111538