biostudies-literatureSun CGuizhou Provincial Natural Science FoundationNational Natural Science Foundation of ChinaScience and Technology Planning Project of Guizhou Province1474https://www.ebi.ac.uk/biostudies/studies/S-EPMC9686669biostudies-literatureUnknown11(11)The biofilm formation of <i>C. albicans</i> represents a major virulence factor during candidiasis. Biofilm-mediated drug resistance has necessitated the search for a new antifungal treatment strategy. In our previous study, a novel antimicrobial peptide named AMP-17 derived from <i>Musca domestica</i> was confirmed to have significant antifungal activity and suppress hyphal growth greatly in <i>C. albicans</i>. In the current work, we aimed to investigate the antibiofilm property of AMP-17 in <i>C. albicans</i> and explore the underlying mechanism. An antifungal susceptibility assay showed that AMP-17 exerted a strong inhibitory efficacy on both biofilm formation and preformed biofilms in <i>C. albicans</i>. Furthermore, AMP-17 was found to block the yeast-to-hypha transition and inhibit Antibiotics (Basel, Switzerland)The Antimicrobial Peptide AMP-17 Derived from <i>Musca domestica</i> Inhibits Biofilm Formation and Eradicates Mature Biofilm in <i>Candida albicans</i>.PMC9686669ZK[2022] general project 345(ZK[2022] Key Program 039)8200218082060381Zhou LYang LSun CGuo GHuang MTian CJiao ZPeng JZhao XfalseThe Antimicrobial Peptide AMP-17 Derived from <i>Musca domestica</i> Inhibits Biofilm Formation and Eradicates Mature Biofilm in <i>Candida albicans</i>.The biofilm formation of <i>C. albicans</i> represents a major virulence factor during candidiasis. Biofilm-mediated drug resistance has necessitated the search for a new antifungal treatment strategy. In our previous study, a novel antimicrobial peptide named AMP-17 derived from <i>Musca domestica</i> was confirmed to have significant antifungal activity and suppress hyphal growth greatly in <i>C. albicans</i>. In the current work, we aimed to investigate the antibiofilm property of AMP-17 in <i>C. albicans</i> and explore the underlying mechanism. An antifungal susceptibility assay showed that AMP-17 exerted a strong inhibitory efficacy on both biofilm formation and preformed biofilms in <i>C. albicans</i>. Furthermore, AMP-17 was found to block the yeast-to-hypha transition and inhibit 2022-01-01T00:00:00Z2022 Oct2025-04-18T17:08:07.131Z2025-04-07T04:43:26.117ZS-EPMC96866693635812910.3390/antibiotics11111474