biostudies-literatureRosiak NNational Science Center2097https://www.ebi.ac.uk/biostudies/studies/S-EPMC9686677biostudies-literatureUnknown11(11)The purpose of this study was to determine the effect of electron beam irradiation (EBI) at a dose of 25 kGy on the stability and antioxidant properties of resveratrol (RSV), a nutraceutical with clinically proven activity. The electron paramagnetic resonance (EPR) method was used to evaluate the concentration of free radicals after irradiation. Minor changes in chemical structure due to free radicals induced by EBI were confirmed by FTIR spectroscopy. HPLC and HPLC-MS analysis ruled out the appearance of degradation products after irradiation. In addition, HPLC analysis confirmed the absence of trans- to cis-resveratrol conversion. Changes in the antioxidant potential of RSV after irradiation were studied using DPPH, ABTS, CUPRAC, and FRAP techniques. It was confirmed that EBI favorably aAntioxidants (Basel, Switzerland)Antioxidant Potential of Resveratrol as the Result of Radiation Exposition.PMC96866772020/37/B/NZ7/03975Zalewski PBednarski WLewandowska KSkibinski RRosiak NCielecka-Piontek JfalseAntioxidant Potential of Resveratrol as the Result of Radiation Exposition.The purpose of this study was to determine the effect of electron beam irradiation (EBI) at a dose of 25 kGy on the stability and antioxidant properties of resveratrol (RSV), a nutraceutical with clinically proven activity. The electron paramagnetic resonance (EPR) method was used to evaluate the concentration of free radicals after irradiation. Minor changes in chemical structure due to free radicals induced by EBI were confirmed by FTIR spectroscopy. HPLC and HPLC-MS analysis ruled out the appearance of degradation products after irradiation. In addition, HPLC analysis confirmed the absence of trans- to cis-resveratrol conversion. Changes in the antioxidant potential of RSV after irradiation were studied using DPPH, ABTS, CUPRAC, and FRAP techniques. It was confirmed that EBI favorably a2022-01-01T00:00:00Z2022 Oct2025-04-25T21:03:52.775Z2025-04-06T08:33:48.738ZS-EPMC96866773635846910.3390/antiox11112097