{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Krishnarjuna B"],"funding":["National Institute of Health","NCI NIH HHS","NIGMS NIH HHS"],"pagination":["1628"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9687133"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["12(11)"],"pubmed_abstract":["Residual dipolar couplings (RDCs) are increasingly used for high-throughput NMR-based structural studies and to provide long-range angular constraints to validate and refine structures of various molecules determined by X-ray crystallography and NMR spectroscopy. RDCs of a given molecule can be measured in an anisotropic environment that aligns in an external magnetic field. Here, we demonstrate the first application of polymer-based nanodiscs for the measurement of RDCs from nucleic acids. Polymer-based nanodiscs prepared using negatively charged SMA-EA polymer and zwitterionic DMPC lipids were characterized by size-exclusion chromatography, <sup>1</sup>H NMR, dynamic light-scattering, and <sup>2</sup>H NMR. The magnetically aligned polymer-nanodiscs were used as an alignment medium to measure RDCs from a <sup>13</sup>C/<sup>15</sup>N-labeled fluoride riboswitch aptamer using 2D ARTSY-HSQC NMR experiments. The results showed that the alignment of nanodiscs is stable for nucleic acids and nanodisc-induced RDCs fit well with the previously determined solution structure of the riboswitch. These results demonstrate that SMA-EA-based lipid-nanodiscs can be used as a stable alignment medium for high-resolution structural and dynamical studies of nucleic acids, and they can also be applicable to study various other biomolecules and small molecules in general."],"journal":["Biomolecules"],"pubmed_title":["Polymer-Nanodiscs as a Novel Alignment Medium for High-Resolution NMR-Based Structural Studies of Nucleic Acids."],"pmcid":["PMC9687133"],"funding_grant_id":["R24GM141526","R24 GM141526","R01 GM114432","R35GM139573","R01GM114432","P30 CA016086","R35 GM139573"],"pubmed_authors":["Krishnarjuna B","Faison EM","Tonelli M","Ravula T","Zhang Q","Ramamoorthy A"],"additional_accession":[]},"is_claimable":false,"name":"Polymer-Nanodiscs as a Novel Alignment Medium for High-Resolution NMR-Based Structural Studies of Nucleic Acids.","description":"Residual dipolar couplings (RDCs) are increasingly used for high-throughput NMR-based structural studies and to provide long-range angular constraints to validate and refine structures of various molecules determined by X-ray crystallography and NMR spectroscopy. RDCs of a given molecule can be measured in an anisotropic environment that aligns in an external magnetic field. Here, we demonstrate the first application of polymer-based nanodiscs for the measurement of RDCs from nucleic acids. Polymer-based nanodiscs prepared using negatively charged SMA-EA polymer and zwitterionic DMPC lipids were characterized by size-exclusion chromatography, <sup>1</sup>H NMR, dynamic light-scattering, and <sup>2</sup>H NMR. The magnetically aligned polymer-nanodiscs were used as an alignment medium to measure RDCs from a <sup>13</sup>C/<sup>15</sup>N-labeled fluoride riboswitch aptamer using 2D ARTSY-HSQC NMR experiments. The results showed that the alignment of nanodiscs is stable for nucleic acids and nanodisc-induced RDCs fit well with the previously determined solution structure of the riboswitch. These results demonstrate that SMA-EA-based lipid-nanodiscs can be used as a stable alignment medium for high-resolution structural and dynamical studies of nucleic acids, and they can also be applicable to study various other biomolecules and small molecules in general.","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Nov","modification":"2026-05-08T03:11:22.189Z","creation":"2025-04-21T14:15:37.685Z"},"accession":"S-EPMC9687133","cross_references":{"pubmed":["36358983"],"doi":["10.3390/biom12111628"]}}