{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Kim MJ"],"funding":["Ministry of Health and Welfare, Korea","Ministry of Health &amp; Welfare, Republic of Korea","Korea Association of Health Promotion","National Research Foundation of Korea"],"pagination":["2812"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9687185"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["10(11)"],"pubmed_abstract":["Toxoplasmosis diagnosis predominantly relies on serology testing via enzyme-linked immunosorbent assay (ELISA), but these results are highly variable. Consequently, various antigens are being evaluated to improve the sensitivity and specificity of toxoplasmosis serological diagnosis. Here, we generated <i>Toxoplasma gondii</i> virus-like particles displaying AMA1 of <i>T. gondii</i> and evaluated their diagnostic potential. We found that AMA1 VLPs were highly sensitive and reacted with the sera acquired from mice infected with either <i>T. gondii</i> ME49 or RH strains. The overall IgG and IgM antibody responses elicited by AMA1 VLPs were substantially higher than those induced by the conventionally used <i>T. gondii</i> lysate antigen (TLA). Importantly, AMA1 VLPs were capable of detecting parasitic infection with <i>T. gondii</i> RH and ME49 as early as 1 week post-infection, even when mice were exposed to low infectious doses (5 × 10<sup>3</sup> and 10 cysts, respectively). AMA1 VLPs also did not cross-react with the immune sera acquired from <i>Plasmodium berghei</i>-infected mice. Compared to TLA, stronger antibody responses were induced by AMA1 VLPs when tested using <i>T. gondii</i>-infected human sera. The sensitivities and specificities of the two antigens were substantially different, with AMA1 VLPs demonstrating over 90% sensitivity and specificity, whereas these values were in the 70% range for the TLA. These results indicated that AMA1 VLPs can detect infections of both <i>T. gondii</i> ME49 and RH at an early stage of infection caused by very low infection doses in mice, and these could be used for serological diagnosis of human toxoplasmosis."],"journal":["Biomedicines"],"pubmed_title":["Recombinant AMA1 Virus-like Particle Antigen for Serodiagnosis of <i>Toxoplasma gondii</i> Infection."],"pmcid":["PMC9687185"],"funding_grant_id":["2018R1A6A1A03025124","2020-01","HV20C0085, HV20C0142","NRF-2018-R1A6A1A03025124","HV20C0142","HV20C0085"],"pubmed_authors":["Chu KB","Yoon KW","Kang HJ","Quan FS","Eom GD","Lee SH","Kim MJ","Moon EK","Lee YH","Mao J"],"additional_accession":[]},"is_claimable":false,"name":"Recombinant AMA1 Virus-like Particle Antigen for Serodiagnosis of <i>Toxoplasma gondii</i> Infection.","description":"Toxoplasmosis diagnosis predominantly relies on serology testing via enzyme-linked immunosorbent assay (ELISA), but these results are highly variable. Consequently, various antigens are being evaluated to improve the sensitivity and specificity of toxoplasmosis serological diagnosis. Here, we generated <i>Toxoplasma gondii</i> virus-like particles displaying AMA1 of <i>T. gondii</i> and evaluated their diagnostic potential. We found that AMA1 VLPs were highly sensitive and reacted with the sera acquired from mice infected with either <i>T. gondii</i> ME49 or RH strains. The overall IgG and IgM antibody responses elicited by AMA1 VLPs were substantially higher than those induced by the conventionally used <i>T. gondii</i> lysate antigen (TLA). Importantly, AMA1 VLPs were capable of detecting parasitic infection with <i>T. gondii</i> RH and ME49 as early as 1 week post-infection, even when mice were exposed to low infectious doses (5 × 10<sup>3</sup> and 10 cysts, respectively). AMA1 VLPs also did not cross-react with the immune sera acquired from <i>Plasmodium berghei</i>-infected mice. Compared to TLA, stronger antibody responses were induced by AMA1 VLPs when tested using <i>T. gondii</i>-infected human sera. The sensitivities and specificities of the two antigens were substantially different, with AMA1 VLPs demonstrating over 90% sensitivity and specificity, whereas these values were in the 70% range for the TLA. These results indicated that AMA1 VLPs can detect infections of both <i>T. gondii</i> ME49 and RH at an early stage of infection caused by very low infection doses in mice, and these could be used for serological diagnosis of human toxoplasmosis.","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Nov","modification":"2025-04-18T20:31:02.554Z","creation":"2025-04-07T08:20:10.654Z"},"accession":"S-EPMC9687185","cross_references":{"pubmed":["36359332"],"doi":["10.3390/biomedicines10112812"]}}