{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Tatsumi A"],"funding":["the Japan Society for the Promotion of Science and Kawano Masanori Memorial Foundation for Promotion of Pediatrics"],"pagination":["1551"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9687188"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["11(11)"],"pubmed_abstract":["Mutations in <i>NRAS</i> constitutively activate cell proliferation signaling in malignant neoplasms, such as leukemia and melanoma, and the clarification of comprehensive downstream genes of <i>NRAS</i> might lead to the control of cell-proliferative signals of <i>NRAS</i>-driven cancers. We previously established that <i>NRAS</i> expression and proliferative activity can be controlled with doxycycline and named as THP-1 B11. Using a CRISPR activation library on THP-1 B11 cells with the <i>NRAS</i>-off state, survival clones were harvested, and 21 candidate genes were identified. By inducting each candidate guide RNA with the CRISPR activation system, <i>DOHH</i>, <i>HIST1H2AC</i>, <i>KRT32</i>, and <i>TAF6</i> showed higher cell-proliferative activity. The expression of <i>DOHH</i>, <i>H"],"journal":["Biology"],"pubmed_title":["Identification of <i>NRAS</i> Downstream Genes with CRISPR Activation Screening."],"pmcid":["PMC9687188"],"funding_grant_id":["18K06953"],"pubmed_authors":["Yamamoto K","Inoue S","Furuno H","Taguchi T","Onishi I","Largaespada DA","Kitagawa M","Tanaka Y","Sachs Z","Kurata M","Ikeda M","Ishibashi S","Tatsumi A","Hirakochi H"],"additional_accession":[]},"is_claimable":false,"name":"Identification of <i>NRAS</i> Downstream Genes with CRISPR Activation Screening.","description":"Mutations in <i>NRAS</i> constitutively activate cell proliferation signaling in malignant neoplasms, such as leukemia and melanoma, and the clarification of comprehensive downstream genes of <i>NRAS</i> might lead to the control of cell-proliferative signals of <i>NRAS</i>-driven cancers. We previously established that <i>NRAS</i> expression and proliferative activity can be controlled with doxycycline and named as THP-1 B11. Using a CRISPR activation library on THP-1 B11 cells with the <i>NRAS</i>-off state, survival clones were harvested, and 21 candidate genes were identified. By inducting each candidate guide RNA with the CRISPR activation system, <i>DOHH</i>, <i>HIST1H2AC</i>, <i>KRT32</i>, and <i>TAF6</i> showed higher cell-proliferative activity. The expression of <i>DOHH</i>, <i>H","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Oct","modification":"2025-04-18T14:59:39.742Z","creation":"2025-04-07T01:24:30.629Z"},"accession":"S-EPMC9687188","cross_references":{"pubmed":["36358254"],"doi":["10.3390/biology11111551"]}}