biostudies-literatureXu XNational Natural Science Foundation of China1602https://www.ebi.ac.uk/biostudies/studies/S-EPMC9687563biostudies-literatureUnknown11(11)The fall armyworm (<i>Spodoptera frugiperda</i>, J.E. Smith) is one of the most important agricultural pests in the world and causes serious damage to many significant crops. Insect gut microbiota plays a vital role in host immunity, digestion, and development, helping the higher organism colonize in a new environment. However, the effects of different diets on midgut microbial composition and host immunity in <i>S. frugiperda</i> remain unclear. So far, no reports have compared the gut microbiota of fall armyworm reared using an artificial diet compared to corn leaf in Guangzhou, China. High-throughput 16S rRNA sequencing technology was applied to gain insight into the composition of the gut microbiota of <i>S. frugiperda</i> feeding on corn leaf (field diet) and on a starch-rich artificiBiologyEffect of Diet on the Midgut Microbial Composition and Host Immunity of the Fall Armyworm, <i>Spodoptera frugiperda</i>.PMC96875633217249831972345Jin FLin SDe Mandal SXu XKong JWu HZhu SfalseEffect of Diet on the Midgut Microbial Composition and Host Immunity of the Fall Armyworm, <i>Spodoptera frugiperda</i>.The fall armyworm (<i>Spodoptera frugiperda</i>, J.E. Smith) is one of the most important agricultural pests in the world and causes serious damage to many significant crops. Insect gut microbiota plays a vital role in host immunity, digestion, and development, helping the higher organism colonize in a new environment. However, the effects of different diets on midgut microbial composition and host immunity in <i>S. frugiperda</i> remain unclear. So far, no reports have compared the gut microbiota of fall armyworm reared using an artificial diet compared to corn leaf in Guangzhou, China. High-throughput 16S rRNA sequencing technology was applied to gain insight into the composition of the gut microbiota of <i>S. frugiperda</i> feeding on corn leaf (field diet) and on a starch-rich artifici2022-01-01T00:00:00Z2022 Nov2025-04-18T14:59:16.69Z2025-04-07T01:24:12.648ZS-EPMC96875633635830310.3390/biology11111602