biostudies-literatureWang ZInnovative Talent Team Program from Education Department of Guizhou ProvinceScience and Technology Foundation of QiandongnanInternational Cooperation Base for Insect Evolutionary Biology and Pest ControlScience and Technology Foundation of Guizhou ProvinceNatural Science Research Project of the Education Department of Guizhou Province1699https://www.ebi.ac.uk/biostudies/studies/S-EPMC9687761biostudies-literatureUnknown12(11)Trehalase (Tre) is a crucial enzyme involved in trehalose metabolism, and it plays pivotal roles in insect development and metamorphosis. However, the biological function of <i>Tre</i> genes in <i>Sogatella furcifera</i> remains unclear. In the present study, two <i>Tre</i> genes-<i>SfTre1</i> and <i>SfTre2</i>-were cloned and identified based on the <i>S. furcifera</i> transcriptome data. Bioinformatic analysis revealed that the full-length complementary DNA of <i>SfTre1</i> and <i>SfTre2</i> genes were 3700 and 2757 bp long, with 1728- and 1902-bp open reading frame encoding 575 and 633 amino acid residues, respectively. Expression analysis indicated that <i>SfTre1</i> and <i>SfTre2</i> were expressed at all developmental stages, with the highest expression in day two adults. FurthermoreBiomoleculesKnockdown of Two Trehalase Genes by RNA Interference Is Lethal to the White-Backed Planthopper <i>Sogatella furcifera</i> (Horvath) (Hemiptera: Delphacidae).PMC9687761201901-2KY202019020132620201Y07820165802J2019108Jin DCYang HLong GYZhou CYang XBWang ZfalseKnockdown of Two Trehalase Genes by RNA Interference Is Lethal to the White-Backed Planthopper <i>Sogatella furcifera</i> (Horvath) (Hemiptera: Delphacidae).Trehalase (Tre) is a crucial enzyme involved in trehalose metabolism, and it plays pivotal roles in insect development and metamorphosis. However, the biological function of <i>Tre</i> genes in <i>Sogatella furcifera</i> remains unclear. In the present study, two <i>Tre</i> genes-<i>SfTre1</i> and <i>SfTre2</i>-were cloned and identified based on the <i>S. furcifera</i> transcriptome data. Bioinformatic analysis revealed that the full-length complementary DNA of <i>SfTre1</i> and <i>SfTre2</i> genes were 3700 and 2757 bp long, with 1728- and 1902-bp open reading frame encoding 575 and 633 amino acid residues, respectively. Expression analysis indicated that <i>SfTre1</i> and <i>SfTre2</i> were expressed at all developmental stages, with the highest expression in day two adults. Furthermore2022-01-01T00:00:00Z2022 Nov2025-04-05T00:44:24.988Z2025-04-05T00:44:24.988ZS-EPMC96877613642171310.3390/biom12111699