biostudies-literatureUnknown12(11)Cortez-Trejo MCPomegranate (PMG; <i>Punica granatum</i> L.) fruits possess a well-balanced nutrient/phytochemical composition, with proven adjuvant benefits in experimental cancer chemotherapy; however, such bioactivity could be affected by PMG's phenogenotype (varietal). Here, the chemical and phytochemical (UPLC-DAD-MS²) composition, antioxidant capacity and anticancer potential [in vitro (MTT assay) and in silico (foodinformatics)] of three PMG fruits of different aryl color [red (cv. Wonderful), pink (cv. Molar de Elche), and white (cv. Indian)] were evaluated. The macro/micronutrient (ascorbic acid, tocols, carotenoids), organic acid (citric/malic), and polyphenol content were changed by PMG's varietal and total antioxidant activity (ABTS, alcoholic &gt; hexane extract) in the order of red &gt; pink &gt; white. However, their in vitro cytotoxicity was the same (IC₅₀ &gt; 200 μg.mL^-1) against normal (retinal) and cancer (breast, lung, colorectal) cell lines. Sixteen major phytochemicals were tentatively identified, four of them with a high GI absorption/bioavailability score [Ellagic (pink), vanillic (red), gallic (white) acids, D-(+)-catechin (white)] and three of them with multiple molecular targets [Ellagic (52) &gt; vanillic (32) &gt; gallic (23)] associated with anticancer (at initiation and promotion stages) activity. The anticancer potential of the PMG fruit is phenogenotype-specific, although it could be more effective in nutraceutical formulations (concentrates).Biomolecules1649https://www.ebi.ac.uk/biostudies/studies/S-EPMC9687934biostudies-literaturePotential Anticancer Activity of Pomegranate (<i>Punica granatum</i> L.) Fruits of Different Color: In Vitro and In Silico Evidence.PMC9687934Dufoo-Hurtado EOlivas-Aguirre FJWall-Medrano ACortez-Trejo MCMendoza SVillegas-Quintero HCastaneda-Moreno RMedina-Franco JLfalsePotential Anticancer Activity of Pomegranate (<i>Punica granatum</i> L.) Fruits of Different Color: In Vitro and In Silico Evidence.Pomegranate (PMG; <i>Punica granatum</i> L.) fruits possess a well-balanced nutrient/phytochemical composition, with proven adjuvant benefits in experimental cancer chemotherapy; however, such bioactivity could be affected by PMG's phenogenotype (varietal). Here, the chemical and phytochemical (UPLC-DAD-MS²) composition, antioxidant capacity and anticancer potential [in vitro (MTT assay) and in silico (foodinformatics)] of three PMG fruits of different aryl color [red (cv. Wonderful), pink (cv. Molar de Elche), and white (cv. Indian)] were evaluated. The macro/micronutrient (ascorbic acid, tocols, carotenoids), organic acid (citric/malic), and polyphenol content were changed by PMG's varietal and total antioxidant activity (ABTS, alcoholic &gt; hexane extract) in the order of red &gt; pink &gt; white. However, their in vitro cytotoxicity was the same (IC₅₀ &gt; 200 μg.mL^-1) against normal (retinal) and cancer (breast, lung, colorectal) cell lines. Sixteen major phytochemicals were tentatively identified, four of them with a high GI absorption/bioavailability score [Ellagic (pink), vanillic (red), gallic (white) acids, D-(+)-catechin (white)] and three of them with multiple molecular targets [Ellagic (52) &gt; vanillic (32) &gt; gallic (23)] associated with anticancer (at initiation and promotion stages) activity. The anticancer potential of the PMG fruit is phenogenotype-specific, although it could be more effective in nutraceutical formulations (concentrates).2022-01-01T00:00:00Z2022 Nov2025-04-05T15:21:37.228Z2025-04-05T15:21:37.228ZS-EPMC96879343635899910.3390/biom12111649