biostudies-literatureWulf MMinistry of Innovation, Science and Research of North-Rhine Westphalia, GermanyGerman Federal Ministry of Education and Research3538https://www.ebi.ac.uk/biostudies/studies/S-EPMC9688080biostudies-literatureUnknown11(22)Neuromelanin granules (NMGs) are organelle-like structures present in the human <i>substantia nigra pars compacta</i>. In addition to neuromelanin, NMGs contain proteins, lipids and metals. As NMG-containing dopaminergic neurons are preferentially lost in Parkinson's disease and dementia with Lewy bodies (DLB), it is assumed that NMGs may play a role in neurodegenerative processes. Until now, this role is not completely understood and needs further investigation. We therefore set up an exploratory proteomic study to identify differences in the proteomic profile of NMGs from DLB patients (n = 5) compared to healthy controls (CTRL, n = 5). We applied a laser microdissection and mass-spectrometry-based approach, in which we used targeted mass spectrometric experiments for validation. In NMG-sCellsThe Proteome of Neuromelanin Granules in Dementia with Lewy Bodies.PMC9688080FKZ 031 A 534AGerlach MMarcus KEggers BBarkovits KEisenacher MWulf MRiederer PSchork KfalseThe Proteome of Neuromelanin Granules in Dementia with Lewy Bodies.Neuromelanin granules (NMGs) are organelle-like structures present in the human <i>substantia nigra pars compacta</i>. In addition to neuromelanin, NMGs contain proteins, lipids and metals. As NMG-containing dopaminergic neurons are preferentially lost in Parkinson's disease and dementia with Lewy bodies (DLB), it is assumed that NMGs may play a role in neurodegenerative processes. Until now, this role is not completely understood and needs further investigation. We therefore set up an exploratory proteomic study to identify differences in the proteomic profile of NMGs from DLB patients (n = 5) compared to healthy controls (CTRL, n = 5). We applied a laser microdissection and mass-spectrometry-based approach, in which we used targeted mass spectrometric experiments for validation. In NMG-s2022-01-01T00:00:00Z2022 Nov2026-05-13T14:24:25.831Z2025-04-06T09:24:58.391ZS-EPMC96880803642896610.3390/cells11223538