biostudies-literatureUnknown12(11)Everitt MLTo combat pandemics, there is a need for rapid point-of-care diagnostics to identify infected patients and to track the spread of the disease. While recent progress has been made in response to COVID-19, there continues to be a need for point-of-care diagnostics capable of detecting biomarkers-such as antibodies-in whole blood. We have recently reported the development of thermally responsive alkane partitions (TRAPs) for the automation of point-of-care immuno-magnetic assays. Here, we demonstrate the use of TRAPs to enable sample-to-answer detection of antibodies against the SARS-CoV-2 virus in whole blood samples. We report a limit of detection of 84 pg/mL, well below the clinically relevant threshold. We anticipate that the TRAP-enabled sample-to-answer immunoassay can be used to track Biosensors1030https://www.ebi.ac.uk/biostudies/studies/S-EPMC9688217biostudies-literatureSample-to-Answer Immuno-Magnetic Assay Using Thermally Responsive Alkane Partitions.PMC9688217Boegner DJWhite IMEveritt MLfalseSample-to-Answer Immuno-Magnetic Assay Using Thermally Responsive Alkane Partitions.To combat pandemics, there is a need for rapid point-of-care diagnostics to identify infected patients and to track the spread of the disease. While recent progress has been made in response to COVID-19, there continues to be a need for point-of-care diagnostics capable of detecting biomarkers-such as antibodies-in whole blood. We have recently reported the development of thermally responsive alkane partitions (TRAPs) for the automation of point-of-care immuno-magnetic assays. Here, we demonstrate the use of TRAPs to enable sample-to-answer detection of antibodies against the SARS-CoV-2 virus in whole blood samples. We report a limit of detection of 84 pg/mL, well below the clinically relevant threshold. We anticipate that the TRAP-enabled sample-to-answer immunoassay can be used to track 2022-01-01T00:00:00Z2022 Nov2025-04-19T16:11:53.445Z2025-04-19T16:11:53.445ZS-EPMC96882173642114810.3390/bios12111030