{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Lin J"],"funding":["Key Research and Development Program of Zhejiang","Natural Science Foundation of Zhejiang Province","Research Funds project of Wenzhou Medical University","National Natural Science Foundation of China","Zhejiang Provincial Science and Technology Innovation Program (New Young Talent Program) for College Students"],"pagination":["2133"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9690185"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["13(11)"],"pubmed_abstract":["Pyroptosis serves a crucial function in various types of ischemia and reperfusion injuries. Oridonin, a tetracycline diterpene derived from <i>Rabdosia rubescens</i>, can significantly inhibit the aggregation of NLRP3-mediated inflammasome. This experiment is aimed at investigating the effect of oridonin on pyroptosis in mice cardiomyocytes. Based on the models of myocardial ischemia/reperfusion (I/R) and hypoxia/reoxygenation (H/R), Evans Blue/TTC double staining, TUNEL staining, and Western blotting were applied to determine the effects of oridonin on myocardial damage, cellular activity and signaling pathways involved in pyroptosis. During I/R and H/R treatments, the extent of gasdermin D-N domains was upregulated in cardiomyocytes. Apart from that, oridonin improved cell survival in vi"],"journal":["Genes"],"pubmed_title":["Oridonin Protects against Myocardial Ischemia-Reperfusion Injury by Inhibiting GSDMD-Mediated Pyroptosis."],"pmcid":["PMC9690185"],"funding_grant_id":["2021R413010","LQ21H020009","82003750","82070446","2019C03012","KYYW202017"],"pubmed_authors":["Lai X","Fan X","Shi S","Zhang Y","Huang W","Ye B","Su L","Lin J","Shao R","Ying M","Zhong L"],"additional_accession":[]},"is_claimable":false,"name":"Oridonin Protects against Myocardial Ischemia-Reperfusion Injury by Inhibiting GSDMD-Mediated Pyroptosis.","description":"Pyroptosis serves a crucial function in various types of ischemia and reperfusion injuries. Oridonin, a tetracycline diterpene derived from <i>Rabdosia rubescens</i>, can significantly inhibit the aggregation of NLRP3-mediated inflammasome. This experiment is aimed at investigating the effect of oridonin on pyroptosis in mice cardiomyocytes. Based on the models of myocardial ischemia/reperfusion (I/R) and hypoxia/reoxygenation (H/R), Evans Blue/TTC double staining, TUNEL staining, and Western blotting were applied to determine the effects of oridonin on myocardial damage, cellular activity and signaling pathways involved in pyroptosis. During I/R and H/R treatments, the extent of gasdermin D-N domains was upregulated in cardiomyocytes. Apart from that, oridonin improved cell survival in vi","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Nov","modification":"2025-04-19T14:44:17.307Z","creation":"2025-04-19T14:44:17.307Z"},"accession":"S-EPMC9690185","cross_references":{"pubmed":["36421808"],"doi":["10.3390/genes13112133"]}}