biostudies-literatureLin JKey Research and Development Program of ZhejiangNatural Science Foundation of Zhejiang ProvinceResearch Funds project of Wenzhou Medical UniversityNational Natural Science Foundation of ChinaZhejiang Provincial Science and Technology Innovation Program (New Young Talent Program) for College Students2133https://www.ebi.ac.uk/biostudies/studies/S-EPMC9690185biostudies-literatureUnknown13(11)Pyroptosis serves a crucial function in various types of ischemia and reperfusion injuries. Oridonin, a tetracycline diterpene derived from <i>Rabdosia rubescens</i>, can significantly inhibit the aggregation of NLRP3-mediated inflammasome. This experiment is aimed at investigating the effect of oridonin on pyroptosis in mice cardiomyocytes. Based on the models of myocardial ischemia/reperfusion (I/R) and hypoxia/reoxygenation (H/R), Evans Blue/TTC double staining, TUNEL staining, and Western blotting were applied to determine the effects of oridonin on myocardial damage, cellular activity and signaling pathways involved in pyroptosis. During I/R and H/R treatments, the extent of gasdermin D-N domains was upregulated in cardiomyocytes. Apart from that, oridonin improved cell survival in viGenesOridonin Protects against Myocardial Ischemia-Reperfusion Injury by Inhibiting GSDMD-Mediated Pyroptosis.PMC96901852021R413010LQ21H02000982003750820704462019C03012KYYW202017Lai XFan XShi SZhang YHuang WYe BSu LLin JShao RYing MZhong LfalseOridonin Protects against Myocardial Ischemia-Reperfusion Injury by Inhibiting GSDMD-Mediated Pyroptosis.Pyroptosis serves a crucial function in various types of ischemia and reperfusion injuries. Oridonin, a tetracycline diterpene derived from <i>Rabdosia rubescens</i>, can significantly inhibit the aggregation of NLRP3-mediated inflammasome. This experiment is aimed at investigating the effect of oridonin on pyroptosis in mice cardiomyocytes. Based on the models of myocardial ischemia/reperfusion (I/R) and hypoxia/reoxygenation (H/R), Evans Blue/TTC double staining, TUNEL staining, and Western blotting were applied to determine the effects of oridonin on myocardial damage, cellular activity and signaling pathways involved in pyroptosis. During I/R and H/R treatments, the extent of gasdermin D-N domains was upregulated in cardiomyocytes. Apart from that, oridonin improved cell survival in vi2022-01-01T00:00:00Z2022 Nov2025-04-19T14:44:17.307Z2025-04-19T14:44:17.307ZS-EPMC96901853642180810.3390/genes13112133