biostudies-literatureUnknown23(22)Merulla AEThe involvement of non-coding RNAs (ncRNAs) in glioblastoma multiforme (GBM) pathogenesis and progression has been ascertained but their cross-talk within GBM cells remains elusive. We previously demonstrated the role of circSMARCA5 as a tumor suppressor (TS) in GBM. In this paper, we explore the involvement of circSMARCA5 in the control of microRNA (miRNA) expression in GBM. By using TaqMan^® low-density arrays, the expression of 748 miRNAs was assayed in U87MG overexpressing circSMARCA5. Differentially expressed (DE) miRNAs were validated through single TaqMan^® assays in: (i) U87MG overexpressing circSMARCA5; (ii) four additional GBM cell lines (A172; CAS-1; SNB-19; U251MG); (iii) thirty-eight GBM biopsies; (iv) twenty biopsies of unaffected brain parenchyma (UC). VaInternational journal of molecular sciences13676https://www.ebi.ac.uk/biostudies/studies/S-EPMC9690846biostudies-literaturecircSMARCA5 Is an Upstream Regulator of the Expression of miR-126-3p, miR-515-5p, and Their mRNA Targets, <i>Insulin-like Growth Factor Binding Protein 2</i> (<i>IGFBP2</i>) and <i>NRAS Proto-Oncogene, GTPase</i> (<i>NRAS</i>) in Glioblastoma.PMC9690846Merulla AECaltabiano RBattaglia RCerto FBroggi GDi Pietro CAltieri RBarbagallo GMVStella MPurrello MCaponnetto ARagusa MBarbagallo DBarbagallo CfalsecircSMARCA5 Is an Upstream Regulator of the Expression of miR-126-3p, miR-515-5p, and Their mRNA Targets, <i>Insulin-like Growth Factor Binding Protein 2</i> (<i>IGFBP2</i>) and <i>NRAS Proto-Oncogene, GTPase</i> (<i>NRAS</i>) in Glioblastoma.The involvement of non-coding RNAs (ncRNAs) in glioblastoma multiforme (GBM) pathogenesis and progression has been ascertained but their cross-talk within GBM cells remains elusive. We previously demonstrated the role of circSMARCA5 as a tumor suppressor (TS) in GBM. In this paper, we explore the involvement of circSMARCA5 in the control of microRNA (miRNA) expression in GBM. By using TaqMan^® low-density arrays, the expression of 748 miRNAs was assayed in U87MG overexpressing circSMARCA5. Differentially expressed (DE) miRNAs were validated through single TaqMan^® assays in: (i) U87MG overexpressing circSMARCA5; (ii) four additional GBM cell lines (A172; CAS-1; SNB-19; U251MG); (iii) thirty-eight GBM biopsies; (iv) twenty biopsies of unaffected brain parenchyma (UC). Va2022-01-01T00:00:00Z2022 Nov2025-04-04T13:28:01.983Z2025-02-19T04:37:21.076ZS-EPMC96908463643015210.3390/ijms232213676