biostudies-literatureUnknown13Holzhausen AFor studying land plant evolution, the establishment and optimization of model organisms representing streptophytic algae, sister to land plants, is essential. Long-term cultivation experiments with <i>Chara braunii</i> S276 were performed over 8 years, since 4 years (Nov. 2018) under constant conditions. Additionally, short-term experiments for optimization of culture conditions were performed with three strains of <i>C. braunii</i> (S276, NIES-1604 and Lausiger Teiche, LaT-2708). Germination success after application of sterilization agents, addition of gibberellic acid and under different incubation conditions with respect to pre-treatment, irradiance regime and substrate was investigated in order to develop protocols for generative cultivation of at least unialgal cultures. The resultiFrontiers in plant science987741https://www.ebi.ac.uk/biostudies/studies/S-EPMC9691404biostudies-literatureEstablishment and optimization of a new model organism to study early land plant evolution: Germination, cultivation and oospore variation of <i>Chara braunii</i> Gmelin, 1826.PMC9691404Schubert HRieth SStingl NRensing SAHolzhausen AKuhn CfalseEstablishment and optimization of a new model organism to study early land plant evolution: Germination, cultivation and oospore variation of <i>Chara braunii</i> Gmelin, 1826.For studying land plant evolution, the establishment and optimization of model organisms representing streptophytic algae, sister to land plants, is essential. Long-term cultivation experiments with <i>Chara braunii</i> S276 were performed over 8 years, since 4 years (Nov. 2018) under constant conditions. Additionally, short-term experiments for optimization of culture conditions were performed with three strains of <i>C. braunii</i> (S276, NIES-1604 and Lausiger Teiche, LaT-2708). Germination success after application of sterilization agents, addition of gibberellic acid and under different incubation conditions with respect to pre-treatment, irradiance regime and substrate was investigated in order to develop protocols for generative cultivation of at least unialgal cultures. The resulti2022-01-01T00:00:00Z20222025-04-04T19:51:35.652Z2025-04-04T19:51:35.652ZS-EPMC96914043643814710.3389/fpls.2022.987741