{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Sun X"],"funding":["National Natural Science Foundation of China"],"pagination":["1023865"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9692129"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["13"],"pubmed_abstract":["Activation of integrins is crucial for recruitment of flowing leukocytes to inflammatory or injured vascular sites, but their spatiotemporal characteristics are incompletely understood. We discovered that β<sub>2</sub>-integrin activation over the entire surface of neutrophils on immobilized P-selectin occurred <i>via</i> mitogen-activated protein kinase (MAPK) or non-MAPK signaling with a minute-level timescale in a force-dependent manner. In flow, MAPK signaling required intracellular Ca<sup>2+</sup> release to activate integrin within 2 min. Integrin activation <i>via</i> non-MAPK signaling occurred first locally in the vicinity of ligated P-selectin glycoprotein ligand-1 (PSGL-1) within sub-seconds, and then over the entire cell surface within 1 min in an extracellular Ca<sup>2+</sup> influx-dependent manner. The transition from a local (but rapid) to global (but slow) activation mode was triggered by ligating the freshly activated integrin. Lipid rafts, moesin, actin, and talin were involved in non-MAPK signaling. Fluid loads had a slight effect on local integrin activation with a second-level timescale, but served as enhancers of global integrin activation."],"journal":["Frontiers in immunology"],"pubmed_title":["Spatiotemporal characteristics of P-selectin-induced β<sub>2</sub> integrin activation of human neutrophils under flow."],"pmcid":["PMC9692129"],"funding_grant_id":["Grant Nos. 11432006 (J.W.), 12072117 (J.W.), 12172137 (Y.F.), 82170566 (B.H.), 82000518 (B.H.)"],"pubmed_authors":["Fang Y","Wu J","Pan Y","Fang J","Ling Y","Li Q","Wang H","Ji Y","Lin J","Sun X","Huang B","Guo P"],"additional_accession":[]},"is_claimable":false,"name":"Spatiotemporal characteristics of P-selectin-induced β<sub>2</sub> integrin activation of human neutrophils under flow.","description":"Activation of integrins is crucial for recruitment of flowing leukocytes to inflammatory or injured vascular sites, but their spatiotemporal characteristics are incompletely understood. We discovered that β<sub>2</sub>-integrin activation over the entire surface of neutrophils on immobilized P-selectin occurred <i>via</i> mitogen-activated protein kinase (MAPK) or non-MAPK signaling with a minute-level timescale in a force-dependent manner. In flow, MAPK signaling required intracellular Ca<sup>2+</sup> release to activate integrin within 2 min. Integrin activation <i>via</i> non-MAPK signaling occurred first locally in the vicinity of ligated P-selectin glycoprotein ligand-1 (PSGL-1) within sub-seconds, and then over the entire cell surface within 1 min in an extracellular Ca<sup>2+</sup> influx-dependent manner. The transition from a local (but rapid) to global (but slow) activation mode was triggered by ligating the freshly activated integrin. Lipid rafts, moesin, actin, and talin were involved in non-MAPK signaling. Fluid loads had a slight effect on local integrin activation with a second-level timescale, but served as enhancers of global integrin activation.","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022","modification":"2025-04-19T17:28:24.818Z","creation":"2025-04-19T17:28:24.818Z"},"accession":"S-EPMC9692129","cross_references":{"pubmed":["36439190"],"doi":["10.3389/fimmu.2022.1023865"]}}