biostudies-literatureWang SChina Agriculture Research System of MOF and MARANational Key R&amp;amp;D Program of ChinaState Key Laboratory of Animal Nutrition ProjectNational Natural Science Foundation of China801https://www.ebi.ac.uk/biostudies/studies/S-EPMC9693499biostudies-literatureUnknown14(11)Citrinin (CIT) is a mycotoxin found in foods and feeds and most commonly discovered in red yeast rice, a food additive made from ordinary rice by fermentation with <i>Monascus</i>. Currently, no enzyme is known to be able to degrade CIT effectively. In this study, it was discovered that manganese peroxidase (<i>Mr</i>MnP) from <i>Moniliophthora roreri</i> could degrade CIT. The degradation appeared to be fulfilled by a combination of direct and indirect actions of the <i>Mr</i>MnP with the CIT. Pure CIT, at a final concentration of 10 mg/L, was completely degraded by <i>Mr</i>MnP within 72 h. One degradation product was identified to be dihydrocitrinone. The toxicity of the CIT-degradation product decreased, as monitored by the increased survival rate of the Caco-2 cells incubated with <i>ToxinsDetoxification of the Mycotoxin Citrinin by a Manganese Peroxidase from <i>Moniliophthora&nbsp;roreri</i>.PMC96934992021YFC21024002004DA125184G210132220103011CARS-412021YFC2103002Zhang WTu TWang SZhang YHuang HLiu BYao BWang XLuo HSu XfalseDetoxification of the Mycotoxin Citrinin by a Manganese Peroxidase from <i>Moniliophthora&nbsp;roreri</i>.Citrinin (CIT) is a mycotoxin found in foods and feeds and most commonly discovered in red yeast rice, a food additive made from ordinary rice by fermentation with <i>Monascus</i>. Currently, no enzyme is known to be able to degrade CIT effectively. In this study, it was discovered that manganese peroxidase (<i>Mr</i>MnP) from <i>Moniliophthora roreri</i> could degrade CIT. The degradation appeared to be fulfilled by a combination of direct and indirect actions of the <i>Mr</i>MnP with the CIT. Pure CIT, at a final concentration of 10 mg/L, was completely degraded by <i>Mr</i>MnP within 72 h. One degradation product was identified to be dihydrocitrinone. The toxicity of the CIT-degradation product decreased, as monitored by the increased survival rate of the Caco-2 cells incubated with <i>2022-01-01T00:00:00Z2022 Nov2026-05-08T03:12:21.959Z2025-02-19T03:23:32.398ZS-EPMC96934993642297410.3390/toxins14110801