biostudies-literatureWang XNatural Science Foundation of Jiangsu Bureau of Science and TechnologyNational Natural Science Foundation of ChinaProgram for Changjiang Scholars and Innovative Research Team in University from the Ministry of Education of ChinaOpen Project Program from the MOE Key Laboratory of Molecular Epigenetics of China1775https://www.ebi.ac.uk/biostudies/studies/S-EPMC9694994biostudies-literatureUnknown12(11)The BAHD acyltransferase family is a unique class of plant proteins that acylates plant metabolites and participates in plant secondary metabolic processes. However, the BAHD members in Lithospermum erythrorhizon remain unknown and uncharacterized. Although the heterologously expressed L. erythrorhizon BAHD family member LeSAT1 in Escherichia coli has been shown to catalyze the conversion of shikonin to acetylshikonin in vitro, its in vivo role remains unknown. In this study, the characterization, evolution, expression patterns, and gene function of LeBAHDs in L. erythrorhizon were explored by bioinformatics and transgenic analysis. We totally identified 73 LeBAHDs in the reference genome of L. erythrorhizon. All LeBAHDs were phylogenLife (Basel, Switzerland)Genome-Wide Identification of LeBAHDs in Lithospermum erythrorhizon and In Vivo Transgenic Studies Confirm the Critical Roles of LeBAHD1/LeSAT1 in the Conversion of Shikonin to Acetylshikonin.PMC9694994316702983197032131970321, 31670298, U1903201, 3177141331771413BK20191254U1903201IRT_14R27without NumberYang HHe CWen ZMa SYang LQi JYin TWang CYang MCai JLai XYang YFazal AHe ZJie WLiu BWang XfalseGenome-Wide Identification of LeBAHDs in Lithospermum erythrorhizon and In Vivo Transgenic Studies Confirm the Critical Roles of LeBAHD1/LeSAT1 in the Conversion of Shikonin to Acetylshikonin.The BAHD acyltransferase family is a unique class of plant proteins that acylates plant metabolites and participates in plant secondary metabolic processes. However, the BAHD members in Lithospermum erythrorhizon remain unknown and uncharacterized. Although the heterologously expressed L. erythrorhizon BAHD family member LeSAT1 in Escherichia coli has been shown to catalyze the conversion of shikonin to acetylshikonin in vitro, its in vivo role remains unknown. In this study, the characterization, evolution, expression patterns, and gene function of LeBAHDs in L. erythrorhizon were explored by bioinformatics and transgenic analysis. We totally identified 73 LeBAHDs in the reference genome of L. erythrorhizon. All LeBAHDs were phylogen2022-01-01T00:00:00Z2022 Nov2026-06-21T03:13:28.761Z2024-10-15T10:29:35.681ZS-EPMC96949943636293010.3390/life12111775